CDC25B antibody (pSer353)
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- Target See all CDC25B Antibodies
- CDC25B (Cell Division Cycle 25 Homolog B (S. Pombe) (CDC25B))
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Binding Specificity
- pSer353
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Reactivity
- Human, Mouse
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This CDC25B antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
- Specificity
- Phospho-CDC25B (Ser353) Antibody detects endogenous levels of CDC25B only when phosphorylated at Serine 353
- Cross-Reactivity
- Human, Mouse (Murine)
- Purification
- The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
- Immunogen
- A synthesized peptide derived from human CDC25B around the phosphorylation site of Serine 353
- Isotype
- IgG
- Top Product
- Discover our top product CDC25B Primary Antibody
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- Application Notes
- WB 1:500-1:2000 IHC 1:50-1:200, IF/ICC 1:100-1:500
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Store at -20 °C.Stable for 12 months from date of receipt
- Expiry Date
- 12 months
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- Target
- CDC25B (Cell Division Cycle 25 Homolog B (S. Pombe) (CDC25B))
- Alternative Name
- CDC25B (CDC25B Products)
- Synonyms
- CDC25B antibody, AI604853 antibody, cell division cycle 25B antibody, cdc25b antibody, CDC25B antibody, Cdc25b antibody
- Background
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Description: Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity.
Gene: CDC25B
- Molecular Weight
- 64kDa
- Gene ID
- 994
- UniProt
- P30305
- Pathways
- Cell Division Cycle, M Phase, Autophagy
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