IRS1 antibody (pSer307)

Catalog No. ABIN6255185

Product Details anti-IRS1 Antibody

Target: IRS1 (Insulin Receptor Substrate 1 (IRS1))

Binding Specificity: pSer307

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IRS1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: Phospho-IRS-1 (Ser307) Antibody detects endogenous levels of IRS-1 only when phosphorylated at Serine 307

Cross-Reactivity: Human, Monkey, Mouse (Murine), Rat (Rattus)

Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Immunogen: A synthesized peptide derived from human IRS-1 around the phosphorylation site of Serine 307

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000 IHC 1:50-1:200 IF/ICC 1:100-1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C.Stable for 12 months from date of receipt

Expiry Date: 12 months

References for anti-IRS1 antibody (ABIN6255185)

Geng, Zhu, Wang, Xie, Li, Wu, Li, Chen, Wang, Meng, Zhang, Chen, Zhong: "P53 modulates hepatic insulin sensitivity through NF-κB and p38/ERK MAPK pathways." in: Biochemical and biophysical research communications, Vol. 495, Issue 3, pp. 2139-2144, (2018) (PubMed).

Geng, Wang, Zhu, Xie, Li, Wu, Zhu, Jiang, Yang, Li, Chen, Wang, Meng, Zhong: "Curcumin suppresses JNK pathway to attenuate BPA-induced insulin resistance in LO2 cells." in: Biomedicine & pharmacotherapy, Vol. 97, pp. 1538-1543, (2018) (PubMed).

Geng, Wang, Zhu, Xie, Li, Wu, Zhu, Jiang, Yang, Li, Chen, Wang, Meng, Zhu, Wu, Huang, Zhong: "Curcumin attenuates BPA-induced insulin resistance in HepG2 cells through suppression of JNK/p38 pathways." in: Toxicology letters, Vol. 272, pp. 75-83, (2017) (PubMed).

Target Details for IRS1

Target: IRS1 (Insulin Receptor Substrate 1 (IRS1))

Alternative Name: IRS-1 (IRS1 Products)

Synonyms: HIRS-1 antibody, G972R antibody, IRS-1 antibody, IRS1IRM antibody, irs1 antibody, irsu antibody, IRS1 antibody, irs-1 antibody, irs1-a antibody, insulin receptor substrate 1 antibody, insulin receptor substrate 2 antibody, insulin receptor substrate 1 S homeolog antibody, IRS1 antibody, Irs1 antibody, irs2 antibody, irs1.S antibody

Background:

Description: May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit (By similarity).

Gene: IRS1

Molecular Weight: 180kDa

Gene ID: 3667

UniProt: P35568

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Hormone Transport, Negative Regulation of Hormone Secretion, Response to Growth Hormone Stimulus, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process