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Cofilin antibody (pSer3)

CFL1 Reactivity: Human, Mouse, Rat WB, ELISA, IHC, IF, ICC Host: Rabbit Polyclonal unconjugated

Western blot analysis of extracts from Mouse muscle tissue,Hela cells,JK cells and Hela+H2o2 cells, using Phospho-Cofilin (Ser3) Antibody (af3232) at 1/1000 dilution. (Cofilin antibody (pSer3))

ABIN6267443 at 1/200 staining human meningeal carcinomatosis(MC) tissue sections by IHC-P. (Cofilin antibody (pSer3))

ABIN6267443 at 1/200 staining human meningeal carcinomatosis(MC) tissue sections by IHC-P. (Cofilin antibody (pSer3))

ABIN6267443 at 1/200 staining human spleen tissue sections by IHC-P. (Cofilin antibody (pSer3))

ABIN6267443 at 1/200 staining human appendiceal tissue sections by IHC-P. (Cofilin antibody (pSer3))

ABIN6267443 at 1/100 staining rat uterine tissue sections by IHC-P. (Cofilin antibody (pSer3))

ABIN6267443 at 1/100 staining mouse testicular tissue sections by IHC-P. (Cofilin antibody (pSer3))

ABIN6267443 at 1/200 staining human liver tissue cells by ICC/IF. (Cofilin antibody (pSer3))

ABIN6267443 at 1/200 staining human duodenum tissue sections by IHC-P. (Cofilin antibody (pSer3))

ABIN6267443 at 1/200 staining human skin tissue sections by IHC-P. (Cofilin antibody (pSer3))

ABIN6267443 at 1/100 staining rat brain tissue sections by IHC-P. (Cofilin antibody (pSer3))

ABIN6267443 at 1/100 staining mouse brain tissue sections by IHC-P. (Cofilin antibody (pSer3))

ABIN6267443 at 1/200 staining human vascular cancer tissue sections by IHC-P. (Cofilin antibody (pSer3))

ABIN6267443 at 1/100 staining rat appendiceal tissue sections by IHC-P. (Cofilin antibody (pSer3))

ABIN6267443 at 1/200 staining human brain tissue sections by IHC-P. (Cofilin antibody (pSer3))

ABIN6267443 at 1/100 staining rat ovarian tissue sections by IHC-P. (Cofilin antibody (pSer3))

ABIN6267443 at 1/200 staining human myosarcoma tissue sections by IHC-P. (Cofilin antibody (pSer3))

ABIN6267443 at 1/200 staining human esophageal carcinoma tissue sections by IHC-P. (Cofilin antibody (pSer3))

ABIN6267443 at 1/200 staining human glioma tissue sections by IHC-P. (Cofilin antibody (pSer3))

ABIN6267443 at 1/200 staining human pancreatic tissue sections by IHC-P. (Cofilin antibody (pSer3))

ABIN6267443 at 1/100 staining rat gastric tissue sections by IHC-P. (Cofilin antibody (pSer3))

ABIN6267443 at 1/100 staining rat kidney tissue sections by IHC-P. (Cofilin antibody (pSer3))

ABIN6267443 at 1/200 staining human seminoma tissue sections by IHC-P. (Cofilin antibody (pSer3))

ABIN6267443 at 1/100 staining mouse kidney tissue sections by IHC-P. (Cofilin antibody (pSer3))

ABIN6267443 at 1/100 staining rat heart tissue sections by IHC-P. (Cofilin antibody (pSer3))

ABIN6267443 at 1/100 staining mouse splenic tissue sections by IHC-P. (Cofilin antibody (pSer3))

ABIN6267443 at 1/100 staining mouse lung tissue sections by IHC-P. (Cofilin antibody (pSer3))

ABIN6267443 at 1/100 staining rat testicular tissue sections by IHC-P. (Cofilin antibody (pSer3))

Western blot analysis of Cofilin phosphorylation expression in COLO205 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. (Cofilin antibody (pSer3))

29 images

Catalog No. ABIN6255180

Datasheet as PDF

Target See all Cofilin (CFL1) Antibodies

Cofilin (CFL1) (Cofilin 1 (CFL1))
Binding Specificity
24

16

16

15

11

9

8

7

7

7

6

6

5

4

3

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
pSer3
Reactivity
141

98

96

6

6

5

4

1

1

1

1

1
Human, Mouse, Rat
Host
146

9

2
Rabbit
Compatible Secondaries
Clonality
147

9
Polyclonal
Conjugate
69

11

11

8

7

5

3

3

3

3

3

3

3

3

3

3

3

2

2

2

2

2

2

1
This Cofilin antibody is un-conjugated
Application
74

65

48

46

41

41

27

27

14

12

7

7

3

3

3

2

2
Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity

Phospho-Cofilin (Ser3) Antibody detects endogenous levels of Cofilin only when phosphorylated at Serine 3.

Predicted Reactivity

Pig,Bovine,Horse,Sheep,Dog

Purification

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Immunogen

A synthesized peptide derived from human Cofilin around the phosphorylation site of Ser3.

Isotype

IgG

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anti-Cofilin 1 (CFL1) (AA 1-166) antibody
CFL1 Reactivity: Human WB, ELISA, IF, IHC (p) Host: Mouse Monoclonal 1A1 unconjugated

anti-Cofilin 1 (CFL1) antibody
KO Validated CFL1 Reactivity: Human WB, IF, IHC (p), ICC, IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-Cofilin 1 (CFL1) (AA 3-161) antibody
CFL1 Reactivity: Human WB, IHC, ICC, IP Host: Rabbit Polyclonal unconjugated

anti-Cofilin 1 (CFL1) (AA 70-98) antibody
CFL1 Reactivity: Human, Mouse WB, FACS Host: Rabbit Polyclonal RB23947 unconjugated

anti-Cofilin 1 (CFL1) (AA 1-100) antibody
CFL1 Reactivity: Human WB Host: Rabbit Polyclonal unconjugated

anti-Cofilin 1 (CFL1) (AA 2-166) antibody
CFL1 Reactivity: Human, Mouse, Rat WB, ELISA, IHC (p) Host: Rabbit Polyclonal unconjugated

anti-Cofilin 1 (CFL1) (AA 4-144) antibody
CFL1 Reactivity: Human ELISA, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-Cofilin 1 (CFL1) (pSer3) antibody
CFL1 Reactivity: Human WB, ELISA, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-Cofilin 1 (CFL1) (AA 4-32), (N-Term) antibody
CFL1 Reactivity: Human, Mouse WB, IHC (p), FACS Host: Rabbit Polyclonal RB20525 unconjugated

anti-Cofilin 1 (CFL1) (AA 4-32) antibody
CFL1 Reactivity: Human, Mouse WB, IHC, IHC (p), FACS Host: Rabbit Polyclonal unconjugated

Application Notes

WB 1:500-1:2000, IHC 1:200, IF/ICC 1:200, ELISA(peptide) 1:20000-1:40000

Restrictions

For Research Use only
Format

Liquid

Concentration

1 mg/mL

Buffer

Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

-20 °C

Storage Comment

Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date

12 months
Target

Cofilin (CFL1) (Cofilin 1 (CFL1))

Alternative Name

CFL1 (CFL1 Products)

Synonyms

cfl1 antibody, xac1 antibody, xac2 antibody, cfl antibody, cb86 antibody, sb:cb86 antibody, zgc:56501 antibody, wu:fb18a04 antibody, wu:fk77b03 antibody, 12/8 antibody, ADF antibody, ADF/cofilin antibody, Adf antibody, CG4254 antibody, Cadf antibody, D-cof antibody, D61 antibody, Dmel\\CG4254 antibody, T8 antibody, Tsr antibody, cofilin antibody, cofilin/ADF antibody, cofilin/actin depolymerizing factor homolog antibody, l(2)03101 antibody, l(2)k03101 antibody, l(2)k05633 antibody, l(2)k13409 antibody, ntf antibody, CFL antibody, AA959946 antibody, Cof antibody, CFL1 antibody, cofilin 1 (non-muscle) L homeolog antibody, cofilin 1 (non-muscle) antibody, cofilin 1 (non-muscle), like antibody, cofilin 1 antibody, twinstar antibody, cofilin antibody, cofilin 1, non-muscle antibody, Contrafreeloading antibody, cfl1.L antibody, cfl1 antibody, cfl1l antibody, CFL1 antibody, tsr antibody, COF1 antibody, TSTA_042790 antibody, Cfl1 antibody, CFL antibody

Background

Description: Binds to F-actin and exhibits  pH -sensitive F-actin depolymerizing activity. Regulates actin cytoskeleton dynamics. Important for normal progress through mitosis and normal cytokinesis. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required for the up-regulation of atypical chemokine receptor ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation (PubMed:11812157, PubMed:15580268, PubMed:21834987, PubMed:23633677). Required for neural tube morphogenesis and neural crest cell migration (By similarity).
Gene: CFL1

Molecular Weight

19kDa

Gene ID

1072

UniProt

P23528

Pathways

Regulation of Cell Size, Tube Formation, Protein targeting to Nucleus, CXCR4-mediated Signaling Events

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