RFP antibody

Catalog No. ABIN6254205

Product Details anti-RFP Antibody

Target: RFP (Red Fluorescent Protein (RFP))

Reactivity: Discosoma

Host: Goat

Clonality: Polyclonal

Conjugate: This RFP antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: RFP Polyclonal Antibody

Specificity: In 293HEK cells transfected with cds plasmid detects a band of 29 kDa by Western blot. This antibody also recognizes very well tdTomato and does not cross-react to GFP (green fluorescent protein).

Cross-Reactivity (Details): Red Fluorescent Protein (dsRed), mCherry, mOrange, mPlum, mRFP, mStrawberry and tdTomato

Purification: epitope affinity purified

Immunogen: Purified recombinant RFP peptide produced in E. coli.

Isotype: IgG

Application Details

Application Notes: Western blot 1:500-1:2,000
Immunofluorescence 1:50-1:500
Immunohistochemistry (paraffin) 1:50-1:500
Immunohistochemistry (frozen) 1:50-1:500

Restrictions: For Research Use only

Handling

Handling Advice: The antibody solution should be gently mixed before use.

Storage: 4 °C/-20 °C

Storage Comment: For continuous use, store at 2-8°C for one-two days. For extended storage, store in -20°C freezer. Working dilution samples should be discarded if not used within 12 hours.

References for anti-RFP antibody (ABIN6254205)

Semerci, Choi, Bajic, Thakkar, Encinas, Depreux, Segil, Groves, Maletic-Savatic: "Lunatic fringe-mediated Notch signaling regulates adult hippocampal neural stem cell maintenance." in: eLife, Vol. 6, (2018) (PubMed).

Tian, Peng, Murugan, Feng, Liu, Eyo, Zhou, Mogilevsky, Wang, Wu: "Chemokine CCL2-CCR2 Signaling Induces Neuronal Cell Death via STAT3 Activation and IL-1β Production after Status Epilepticus." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 37, Issue 33, pp. 7878-7892, (2017) (PubMed).

Target Details for RFP

Target: RFP (Red Fluorescent Protein (RFP))

Alternative Name: RFP (RFP Products)