EGFR antibody (C-Term)

Catalog No. ABIN6254171

The Goat Polyclonal anti-EGFR antibody is suitable to detect EGFR in samples from Human, Mouse, Rat, Monkey, Pig, Cow, Chicken, Dog, Horse, Rabbit, Guinea Pig, Avian, Cat, Donkey, Goat, Hamster and Sheep. It has been validated for WB, IHC (p) and IHC (fro).

Quick Overview for EGFR antibody (C-Term) (ABIN6254171)

Target: EGFR (Epidermal Growth Factor Receptor (EGFR))

Reactivity: Human, Mouse, Rat, Monkey, Pig, Cow, Chicken, Dog, Horse, Rabbit, Guinea Pig, Avian, Cat, Donkey, Goat, Hamster, Sheep

Host: Goat

Clonality: Polyclonal

Conjugate: This EGFR antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-EGFR Antibody

Binding Specificity: C-Term

Purpose: anti-ERBB1

Sequence: SHQISLDNPD YQQDFFPKEA KPNGIFKGST AENAEYLRVA PQSSEFIGA

Specificity: This antibody gives a positive signal in the following human (HaCat, SKOV3 and H69) whole cell lysates by Western blot.

Characteristics: Epidermal growth factor receptor

Purification: Epitope affinity purified

Immunogen: Purified recombinant peptide derived from within residues 1,162 aa to the C-terminus of human ERBB1 produced in E. coli.

Isotype: IgG

Application Details

Application Notes: WB:1:500-1:5,000, IHC-P:1:250-1:1,000, IHC-F:1:250-1:1,000

Restrictions: For Research Use only

Handling

Buffer: PBS, 20 % glycerol and 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody solution should be gently mixed before use.

Storage: 4 °C,-20 °C

Storage Comment: For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours.

Target Details for EGFR

Target: EGFR (Epidermal Growth Factor Receptor (EGFR))

Alternative Name: ERBB1

Background:

Goat polyclonal antibody to ERBB1. ERBB1 is a transmembrane glycoprotein that is a member of the protein kinase superfamily and a receptor for members of the epidermal growth factor family. It is a cell surface protein that binds to epidermal growth factor inducing receptor dimerization and tyrosine autophosphorylation and leading to cell proliferation. This receptor has been associated with cancer. Different protein isoforms encoded by multiple alternatively spliced transcript variants have been found for this gene.

EGFR, epidermal growth factor receptor, ERBB, HER1, mENA, NISBD2PIG61, antibody.

Pathways: NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation, S100 Proteins