Optimal working dilution should be determined by the investigator.
Restrictions
For Research Use only
Buffer
100 μL in 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.
Storage
-20 °C
Kwon, Neupane, Shin, Gwon, Yin, Shin, Shin, Hong, Park, Yi, Kim, Kang: "Calpain-2 as a Treatment Target in Prenatal Stress-induced Epileptic Spasms in Infant Rats." in: Experimental neurobiology, Vol. 28, Issue 4, pp. 529-536, (2019) (PubMed).
Huang, Grau: "Ionic plasticity and pain: The loss of descending serotonergic fibers after spinal cord injury transforms how GABA affects pain." in: Experimental neurology, Vol. 306, pp. 105-116, (2019) (PubMed).
Mahadevan, Khademullah, Dargaei, Chevrier, Uvarov, Kwan, Bagshaw, Pawson, Emili, De Koninck, Anggono, Airaksinen, Woodin: "Native KCC2 interactome reveals PACSIN1 as a critical regulator of synaptic inhibition." in: eLife, Vol. 6, (2018) (PubMed).
Mahadevan, Dargaei, Ivakine, Hartmann, Ng, Chevrier, Ormond, Nothwang, McInnes, Woodin: "Neto2-null mice have impaired GABAergic inhibition and are susceptible to seizures." in: Frontiers in cellular neuroscience, Vol. 9, pp. 368, (2015) (PubMed).
Toda, Ishida, Kiyama, Yamashita, Lee: "Down-regulation of KCC2 expression and phosphorylation in motoneurons, and increases the number of in primary afferent projections to motoneurons in mice with post-stroke spasticity." in: PLoS ONE, Vol. 9, Issue 12, pp. e114328, (2015) (PubMed).
Target
KCC2 (SLC12A5)
(Solute Carrier Family 12 (Potassium-Chloride Transporter) Member 5 (SLC12A5))