FMR1 antibody (pSer499)

Catalog No. ABIN6254033

Product Details anti-FMR1 Antibody

Target: FMR1 (Fragile X Mental Retardation 1 (FMR1))

Binding Specificity: pSer499

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FMR1 antibody is un-conjugated

Application: Western Blotting (WB)

Cross-Reactivity: Mouse (Murine), Rat (Rattus)

Predicted Reactivity: bovine, canine, chicken, human, mouse, non-human primates, sheep, Xenopus, zebra fish

Purification: Antigen Affinity Purified from Pooled Serum

Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser499 conjugated to KLH

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: 100 μL in 10 mM HEPES (  pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

Storage: -20 °C

References for anti-FMR1 antibody (ABIN6254033)

Bartley, OKeefe, Blice-Baum, Mihailescu, Gong, Miyares, Karaca, Bordey: "Mammalian FMRP S499 Is Phosphorylated by CK2 and Promotes Secondary Phosphorylation of FMRP." in: eNeuro, Vol. 3, Issue 6, (2017) (PubMed).

Reynolds, Smith, Jefferson, Lugo: "Comparison of Equivalence between Two Commercially Available S499-Phosphorylated FMRP Antibodies in Mice." in: PLoS ONE, Vol. 10, Issue 11, pp. e0143134, (2016) (PubMed).

Guimarães-Souza, Perche, Morgans, Duvoisin, Calaza: "Fragile X Mental Retardation Protein expression in the retina is regulated by light." in: Experimental eye research, Vol. 146, pp. 72-82, (2016) (PubMed).

Bartley, OKeefe, Bordey: "FMRP S499 is phosphorylated independent of mTORC1-S6K1 activity." in: PLoS ONE, Vol. 9, Issue 5, pp. e96956, (2015) (PubMed).

Target Details for FMR1

Target: FMR1 (Fragile X Mental Retardation 1 (FMR1))

Alternative Name: FMR1 (FMR1 Products)

Molecular Weight: '71 kDa

Gene ID: 24948

UniProt: Q80WE1

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development