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Igk antibody

Igk Reactivity: Human FACS, IHC, WB, IF Host: Mouse Monoclonal SPM558 unconjugated
Catalog No. ABIN6252720
  • Target See all Igk products
    Igk (Immunoglobulin kappa Chain Complex (Igk))
    Reactivity
    • 5
    • 1
    Human
    Host
    • 4
    • 1
    • 1
    Mouse
    Clonality
    • 4
    • 2
    Monoclonal
    Conjugate
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Igk antibody is un-conjugated
    Application
    • 6
    • 5
    • 3
    • 2
    • 1
    • 1
    Flow Cytometry (FACS), Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF)
    Purification
    Protein G affinity chromatography
    Immunogen
    Recombinant human Ig kappa chain was used as the immunogen for this anti-Kappa antibody.
    Clone
    SPM558
    Isotype
    IgG1 kappa
  • Application Notes
    Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

    Immunofluorescence: 0.5-1 μg/mL

    Western blot: 0.5-1 μg/mL

    Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)

    Prediluted format : incubate for 30 min at RT (2)

    The optimal dilution of the anti-Kappa antibody for each application should be determined by the researcher.

    1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate Buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.2 mg/mL
    Buffer
    PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    Aliquot and Store at -20C. Avoid freez-thaw cycles.
  • Target
    Igk (Immunoglobulin kappa Chain Complex (Igk))
    Alternative Name
    Kappa (Igk Products)
    Synonyms
    kappa antibody, immunoglobulin kappa chain complex antibody, Igk antibody
    Background
    This mAb is specific to kappa light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. The ratio of Kappa to Lambda is 70:30. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the kappa light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant.
    Gene ID
    3514
    UniProt
    P01601, P01834
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