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Melanoma antibody

Reactivity: Human, Horse IF, IHC, FACS Host: Mouse Monoclonal NKI-beteb unconjugated
Catalog No. ABIN6252230
  • Target See all Melanoma products
    Melanoma
    Reactivity
    • 4
    • 2
    • 2
    • 1
    Human, Horse
    Host
    • 4
    Mouse
    Clonality
    • 4
    Monoclonal
    Conjugate
    • 4
    This Melanoma antibody is un-conjugated
    Application
    • 4
    • 3
    • 1
    • 1
    Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FACS)
    Specificity
    Does not react with rat
    Purification
    Protein G affinity chromatography
    Immunogen
    Membranes from a human melanoma metastasis were used as the immunogen for this Melanoma antibody (gp100).
    Clone
    NKI-beteb
    Isotype
    IgG2b kappa
  • Application Notes
    Flow Cytometry: 0.5-1 μg/million cells

    IF: 1-2 μg/mL

    IHC (FFPE): 1-2 μg/mL for 30 minutes at RT (1)

    Differences in protocols and secondaries may require the gp100 antibody to be titered up or down for optimal performance.

    1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.2 mg/mL
    Buffer
    PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    Aliquot and Store at -20C. Avoid freez-thaw cycles.
  • Target
    Melanoma
    Abstract
    Melanoma Products
    Background
    By immunohistochemistry, this melanoma gp100 antibody specifically recognizes a protein in melanocytes and melanomas. It reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin. The melanoma antibody labels formalin/paraffin melanomas and other tumors showing melanocytic differentiation.
    Gene ID
    6490
    UniProt
    P40967
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