Melanoma antibody

Catalog No. ABIN6252230

Product Details anti-Melanoma Antibody

Target: Melanoma

Reactivity: Human, Horse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Melanoma antibody is un-conjugated

Application: Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Specificity: Does not react with rat

Purification: Protein G affinity chromatography

Immunogen: Membranes from a human melanoma metastasis were used as the immunogen for this Melanoma antibody (gp100).

Clone: NKI-beteb

Isotype: IgG2b kappa

Application Details

Application Notes: Flow Cytometry: 0.5-1 μg/million cells

IF: 1-2 μg/mL

IHC (FFPE): 1-2 μg/mL for 30 minutes at RT (1)

Differences in protocols and secondaries may require the gp100 antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Target Details for Melanoma

Target: Melanoma

Abstract: Melanoma Products

Background: By immunohistochemistry, this melanoma gp100 antibody specifically recognizes a protein in melanocytes and melanomas. It reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin. The melanoma antibody labels formalin/paraffin melanomas and other tumors showing melanocytic differentiation.

Gene ID: 6490

UniProt: P40967