Nuclear Marker antibody
-
- Target See all Nuclear Marker products
- Nuclear Marker
-
Reactivity
- Human, Mouse, Rat
-
Host
- Mouse
-
Clonality
- Monoclonal
-
Conjugate
- This Nuclear Marker antibody is un-conjugated
-
Application
- Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
- Specificity
- Does not react with Pig
- Purification
- Protein G affinity chromatography
- Immunogen
- Nuclei of HL60 cells were used as the immunogen for the Nuclear marker antibody.
- Clone
- NM106
- Isotype
- IgG1 kappa
-
-
- Application Notes
-
Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 0.5-1 μg/mL
Immunocytochemistry (Acetone-fixed): 0.25-0.5 μg/mL for 30 min at RT
Immunohistochemistry (FFPE): 0.25-0.5 μg/mL for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)
Optimal dilution of the Nuclear marker antibody should be determined by the researcher.1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. - Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 0.2 mg/mL
- Buffer
- PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Aliquot and Store at -20C. Avoid freez-thaw cycles.
-
- Target
- Nuclear Marker
- Abstract
- Nuclear Marker Products
- Background
- This mAb is an excellent marker for all nuclei in cells in tissues. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. This mAb recognizes an antigen associated with the nuclei in all cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions. It produces a speckled pattern in normal and malignant cells and may be used to stain the nuclei of cells in fixed tissue sections.
-