FAT Atypical Cadherin 2 (FAT2) antibody

Catalog No. ABIN6251792

Product Details

Target: FAT Atypical Cadherin 2 (FAT2)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (IHC), Western Blotting (WB)

Purification: Protein G affinity chromatography

Immunogen: A recombinant human Protocadherin Fat 2 fusion protein was used as the immunogen for this FAT2 antibody.

Clone: 8C5

Isotype: IgG1 kappa

Application Details

Application Notes: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

Immunofluorescence: 1-2 μg/mL

IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT

Titering of the FAT2 antibody may be required for optimal performance.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Target Details

Target: FAT Atypical Cadherin 2 (FAT2)

Alternative Name: FAT2 (FAT2 Products)

Synonyms: EMI2 antibody, Fath2 antibody, Gm523 antibody, mKIAA0811 antibody, CDHF8 antibody, CDHR9 antibody, HFAT2 antibody, MEGF1 antibody, FAT atypical cadherin 2 antibody, Fat2 antibody, FAT2 antibody

Background: Recognizes a protein of 480  kDa, which is identified as FAT2. The cadherins represent a family of Ca2+-dependent adhesion molecules that function to mediate cell-to-cell binding that is critical for the maintenance of structure and morphogenesis. Cadherins each contain a large extracellular domain at the N-terminus, which is characterized by a series of five homologous repeats, the most distal of which is thought to be responsible for binding specificity. The relatively short C-terminal intracellular domain interacts with a variety of cytoplasmic proteins, including beta-catenin, to regulate cadherin function. The cadherin superfamily includes cadherins, protocadherins, desmogleins and desmocollins. FAT2 (FAT tumor suppressor homolog 2) is a single-pass type I membrane protein that belongs to the protocadherin subfamily of cadherins. FAT2 contains one Laminin G-like domain, two EGF-like domains and 32 cadherin domains and is believed to function as a cell adhesion molecule, controlling cell proliferation and playing an important role in cerebellum development.

Gene ID: 2196

UniProt: Q9NYQ8