Ku70 + Ku80 antibody
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- Target See all Ku70 + Ku80 (KU80) products
- Ku70 + Ku80 (KU80) (CG18801 Gene Product From Transcript CG18801-RA (KU80))
- Reactivity
- Human, Primate
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This Ku70 + Ku80 antibody is un-conjugated
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Application
- Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC)
- Specificity
- Does not react with mouse, rat, chicken
- Purification
- Protein G purified monoclonal antibody
- Immunogen
- Nuclear extract of human HL-60 cells was used as the immunogen for this Ku70 + Ku80 antibody.
- Clone
- KU729
- Isotype
- IgG1 kappa
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anti-CG18801 Gene Product From Transcript CG18801-RA (KU80) antibody (PE)
KU80 Reactivity: Human, Primate FACS, IF Host: Mouse Monoclonal KU729 PE
anti-CG18801 Gene Product From Transcript CG18801-RA (KU80) antibodyKU80 Reactivity: Human, Primate FACS, IF, ICC Host: Mouse Monoclonal KU729 unconjugated
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- Application Notes
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Flow cytometry: 0.5-1 μg/10e6 cells
IF: 0.5-1.0 μg/mL
Immunocytochemistry (Acetone-fixed cells): 0.25-0.5 μg/mL for 30 minutes at RT
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Ku70 + Ku80 antibody to be titered up or down for optimal performance. - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.2 mg/mL
- Buffer
- PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Aliquot and Store at -20C. Avoid freez-thaw cycles.
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- Target
- Ku70 + Ku80 (KU80) (CG18801 Gene Product From Transcript CG18801-RA (KU80))
- Alternative Name
- Ku70 + Ku80 (KU80 Products)
- Background
- This antibody recognizes a dimer of two proteins of 70 kDa (Ku70) and ~80 kDa (Ku80), identified as two subunits of Ku. Antbody KU729 recognizes a conformational epitope of the Ku70 + Ku80 dimer, which is destroyed during Western blotting. The Ku70 + Ku80 dimer is important for function of a 460 kDa DNA-dependent protein kinase. Ku protein plays a role in cell signaling, proliferation, DNA repair, replication, transcriptional activation, and apoptosis.
- Gene ID
- 2547
- UniProt
- Q6FG89
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