SMURF2 antibody

Catalog No. ABIN6243825

Product Details anti-SMURF2 Antibody

Target: SMURF2 (SMAD Specific E3 Ubiquitin Protein Ligase 2 (SMURF2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SMURF2 antibody is un-conjugated

Application: Western Blotting (WB)

Purification: This antibody is purified through a protein G column, followed by dialysis against PBS.

Immunogen: This SMURF2 antibody is generated from a mouse immunized with a recombinant protein of human SMURF2.

Clone: 1826CT865-51-28

Isotype: IgG1 kappa

Application Details

Application Notes: WB: 1:2000-1:4000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified monoclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 6 months

Target Details for SMURF2

Target: SMURF2 (SMAD Specific E3 Ubiquitin Protein Ligase 2 (SMURF2))

Alternative Name: SMURF2 (SMURF2 Products)

Synonyms: SMURF2 antibody, 2810411E22Rik antibody, AI558114 antibody, AI649275 antibody, RGD1310067 antibody, wu:fb82a11 antibody, wu:fc41c02 antibody, SMAD specific E3 ubiquitin protein ligase 2 antibody, SMAD specific E3 ubiquitin protein ligase 2 S homeolog antibody, SMURF2 antibody, smurf2 antibody, Smurf2 antibody, smurf2.S antibody

Background: E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Interacts with SMAD1 and SMAD7 in order to trigger their ubiquitination and proteasome-dependent degradation. In addition, interaction with SMAD7 activates autocatalytic degradation, which is prevented by interaction with SCYE1. Forms a stable complex with the TGF-beta receptor-mediated phosphorylated SMAD2 and SMAD3. In this way, SMAD2 may recruit substrates, such as SNON, for ubiquitin-mediated degradation. Enhances the inhibitory activity of SMAD7 and reduces the transcriptional activity of SMAD2. Coexpression of SMURF2 with SMAD1 results in considerable decrease in steady-state level of SMAD1 protein and a smaller decrease of SMAD2 level.

Molecular Weight: 86196

UniProt: Q9HAU4