CHMP3 antibody (N-Term)

Catalog No. ABIN6243582

Product Details anti-CHMP3 Antibody

Target: CHMP3 (Charged Multivesicular Body Protein 3 (CHMP3))

Binding Specificity: AA 28-61, N-Term

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CHMP3 antibody is un-conjugated

Application: Western Blotting (WB)

Predicted Reactivity: B, Pr, Rat

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This CHMP3 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 28-61 amino acids from the N-terminal region of human CHMP3.

Clone: RB51722

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. WB: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 6 months

Target Details for CHMP3

Target: CHMP3 (Charged Multivesicular Body Protein 3 (CHMP3))

Alternative Name: CHMP3 (CHMP3 Products)

Background: Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. Selectively binds to phosphatidylinositol 3,5-bisphosphate PtdIns(3,5)P2 and PtdIns(3,4)P2 in preference to other phosphoinositides tested. Involved in late stages of cytokinesis. Plays a role in endosomal sorting/trafficking of EGF receptor. Isoform 2 prevents stress- mediated cell death and accumulation of reactive oxygen species when expressed in yeast cells.

Molecular Weight: 25073

UniProt: Q9Y3E7