Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Predicted Reactivity
B, Rat
Purification
This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen
This ATG9A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 723~753 amino acids surrounding S738 of human APG9L1.
Apg9 plays a direct role in the formation of the cytoplasm to vacuole targeting and autophagic vesicles, possibly serving as a marker for a specialized compartment essential for these vesicle-mediated alternative targeting pathways. Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole).