POLA1 antibody (AA 1406-1439)

Catalog No. ABIN6243169

Product Details anti-POLA1 Antibody

Target: POLA1 (DNA Polymerase alpha (POLA1))

Binding Specificity: AA 1406-1439

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLA1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Predicted Reactivity: H

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This POLA1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 1406-1439 amino acids from the human region of human POLA1.

Clone: RB57637

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:2000. FC: 1:25

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 6 months

Target Details for POLA1

Target: POLA1 (DNA Polymerase alpha (POLA1))

Alternative Name: POLA1 (POLA1 Products)

Synonyms: pola antibody, AW321876 antibody, Pola antibody, POLA antibody, p180 antibody, polymerase (DNA directed), alpha 1, catalytic subunit S homeolog antibody, polymerase (DNA directed), alpha 1 antibody, DNA polymerase alpha 1, catalytic subunit antibody, pola1.S antibody, Pola1 antibody, POLA1 antibody

Background: Plays an essential role in the initiation of DNA replication. During the S phase of the cell cycle, the DNA polymerase alpha complex (composed of a catalytic subunit POLA1/p180, a regulatory subunit POLA2/p70 and two primase subunits PRIM1/p49 and PRIM2/p58) is recruited to DNA at the replicative forks via direct interactions with MCM10 and WDHD1. The primase subunit of the polymerase alpha complex initiates DNA synthesis by oligomerising short RNA primers on both leading and lagging strands. These primers are initially extended by the polymerase alpha catalytic subunit and subsequently transferred to polymerase delta and polymerase epsilon for processive synthesis on the lagging and leading strand, respectively. The reason this transfer occurs is because the polymerase alpha has limited processivity and lacks intrinsic 3' exonuclease activity for proofreading error, and therefore is not well suited for replicating long complexes.

Molecular Weight: 165913

UniProt: P09884

Pathways: SARS-CoV-2 Protein Interactome