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Sonic Hedgehog antibody (N-Term)

Name: Sonic Hedgehog antibody (N-Term)
SKU: ABIN6242830
Price: 451 USD
Availability: InStock

SHH Reactivity: Human, Mouse, Rat WB, IF, IHC (p), FACS Host: Rabbit Polyclonal RB52387 unconjugated

All lanes : Anti-Shh Antibody (N-term) at 1:2000 dilution Lane 1: F9 whole cell lysate Lane 2: mouse stomach lysates Lane 3: rat liver whole cell lysates Lane 4: NIH/3T3 lysates Lysates/proteins at 20 μg per lane. (Sonic Hedgehog antibody (N-Term))

Overlay histogram showing HT-29 cells stained with (ABIN6242830 and ABIN6577992) (green line). (Sonic Hedgehog antibody (N-Term))

FACS

Immunofluorescent analysis of 4 % paraformaldehyde-fixed, 0. (Sonic Hedgehog antibody (N-Term))

IHC (p)

4 images

Catalog No. ABIN6242830

Datasheet as PDF

Target See all Sonic Hedgehog (SHH) Antibodies

Sonic Hedgehog (SHH)
Binding Specificity
15

11

9

6

4

4

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1
AA 58-91, N-Term
Reactivity
68

51

36

17

10

6

6

5

3

2

2

1

1

1

1

1

1

1
Human, Mouse, Rat
Host
74

13

8

1

1
Rabbit
Compatible Secondaries
Clonality
67

30
Polyclonal
Conjugate
54

8

4

3

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This Sonic Hedgehog antibody is un-conjugated
Application
71

29

25

14

13

10

9

7

7

6

5

4

3

1
Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen

This mouse Shh antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 58-91 amino acids from the N-terminal region of mouse Shh.

Clone

RB52387

Isotype

Ig Fraction

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anti-Sonic Hedgehog (SHH) (N-Term) antibody
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SHH Reactivity: Human, Mouse, Monkey WB, IHC, ELISA, FACS Host: Mouse Monoclonal unconjugated

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SHH Reactivity: Human WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

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SHH Reactivity: Human WB, IHC, IP, ICC Host: Mouse Monoclonal D6 unconjugated

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SHH Reactivity: Human, Rat, Dog, Monkey WB, IF Host: Mouse Monoclonal 10H6 unconjugated

anti-Sonic Hedgehog (SHH) (AA 237-399) antibody
SHH Reactivity: Rat WB, IHC, ICC Host: Rabbit Polyclonal unconjugated

anti-Sonic Hedgehog (SHH) (AA 203-218), (N-Term) antibody
SHH Reactivity: Human, Rat WB, IHC (p) Host: Rabbit Polyclonal unconjugated

Application Notes

IF: 1:25. WB: 1:2000. IHC-P: 1:25. FC: 1:25

Restrictions

For Research Use only
Format

Liquid

Buffer

Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C,-20 °C

Expiry Date

6 months
Target

Sonic Hedgehog (SHH)

Alternative Name

Shh (SHH Products)

Molecular Weight

47773

UniProt

Q62226

Pathways

Hedgehog Signaling, Dopaminergic Neurogenesis, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development