BRCA2 antibody (AA 251-495)

Catalog No. ABIN6242777

Product Details anti-BRCA2 Antibody

Target: BRCA2 (Breast Cancer 2, Early Onset (BRCA2))

Binding Specificity: AA 251-495

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This BRCA2 antibody is un-conjugated

Application: Western Blotting (WB)

Purification: This antibody is purified through a protein G column, followed by dialysis against PBS.

Immunogen: This BRCA2 antibody is generated from a mouse immunized with a recombinant protein between 251-495 amino acids from human BRCA2.

Clone: 1643CT739-91-87

Isotype: IgG1 kappa

Application Details

Application Notes: WB: 1:2000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified monoclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 6 months

Target Details for BRCA2

Target: BRCA2 (Breast Cancer 2, Early Onset (BRCA2))

Alternative Name: BRCA2 (BRCA2 Products)

Background: Involved in double-strand break repair and/or homologous recombination. Binds RAD51 and potentiates recombinational DNA repair by promoting assembly of RAD51 onto single-stranded DNA (ssDNA). Acts by targeting RAD51 to ssDNA over double-stranded DNA, enabling RAD51 to displace replication protein-A (RPA) from ssDNA and stabilizing RAD51-ssDNA filaments by blocking ATP hydrolysis. Part of a PALB2-scaffolded HR complex containing RAD51C and which is thought to play a role in DNA repair by HR. May participate in S phase checkpoint activation. Binds selectively to ssDNA, and to ssDNA in tailed duplexes and replication fork structures. May play a role in the extension step after strand invasion at replication-dependent DNA double-strand breaks, together with PALB2 is involved in both POLH localization at collapsed replication forks and DNA polymerization activity. In concert with NPM1, regulates centrosome duplication. Interacts with the TREX-2 complex (transcription and export complex 2) subunits PCID2 and DSS1, and is required to prevent R-loop- associated DNA damage and thus transcription-associated genomic instability. Silencing of BRCA2 promotes R-loop accumulation at actively transcribed genes in replicating and non-replicating cells, suggesting that BRCA2 mediates the control of R-loop associated genomic instability, independently of its known role in homologous recombination (PubMed:< a href="http://www.uniprot.org/citations/24896180" target="_blank">24896180).

Molecular Weight: 384202

UniProt: P51587

Pathways: DNA Damage Repair, M Phase, Maintenance of Protein Location