RAD9A antibody (N-Term)

Catalog No. ABIN6242554

Product Details anti-RAD9A Antibody

Target: RAD9A (RAD9 Homolog A (S. Pombe) (RAD9A))

Binding Specificity: AA 29-62, N-Term

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAD9A antibody is un-conjugated

Application: Western Blotting (WB)

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This mouse Rad9a antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 29-62 amino acids from the N-terminal region of mouse Rad9a.

Clone: RB52362

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:2000. WB: 1:2000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 6 months

Target Details for RAD9A

Target: RAD9A (RAD9 Homolog A (S. Pombe) (RAD9A))

Alternative Name: Rad9a (RAD9A Products)

Synonyms: RAD9 antibody, Rad9 antibody, rad9a antibody, MGC89062 antibody, XRad9 antibody, rad9 antibody, RAD9 checkpoint clamp component A antibody, RAD9 checkpoint clamp component B antibody, RAD9 checkpoint clamp component A L homeolog antibody, RAD9A antibody, Rad9a antibody, rad9a antibody, rad9b antibody, rad9a.L antibody

Background: Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds, endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths, and DNA ligase I (LIG1) on long-patch base excision repair substrates. The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase. RAD9A possesses 3'->5' double stranded DNA exonuclease activity (By similarity).

Molecular Weight: 42059

UniProt: Q9Z0F6

Pathways: Positive Regulation of Response to DNA Damage Stimulus