HSP18.5 antibody
HSP18.5-C
Reactivity: Arabidopsis thaliana
WB
Host: Rabbit
Polyclonal
unconjugated
-
- Target
- HSP18.5 (HSP18.5-C) (18.5 kDa Class I Heat Shock Protein (HSP18.5-C))
- Reactivity
- Arabidopsis thaliana
- Host
- Rabbit
- Clonality
- Polyclonal
- Application
- Western Blotting (WB)
- Cross-Reactivity (Details)
- Not reactive in: no confirmed exceptions from predicted reactivity are currently known
- Predicted Reactivity
- dicots including: Glycine max, Medicago truncatula, Medicago sativa, Pisum sativum, Ricinus communis, Rosa chinensis, monocots including: Zea mays
- Characteristics
- Expected / apparent Molecular Weight of the Antigene: 18.5 kDa
- Purification
- serum
- Immunogen
- recombinant Arabidopsis thaliana Hsp18.5
-
- Application Notes
- 1 : 1000 with standard ECL (WB)
- Comment
-
hsp 18.5 is a low abundancy protein and estimated concentration of this protein in total cell extract is ca. 0.007-0.01 %. Therefore to be able to visualize this protein the load per well needs to be at least 20 ug of heat shocked total protein/well. It is crucial to heat treat the plants at 38°C for 3 hours at high humidity.Please, note that longer transfer time might result in losing a signal for this protein.
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Reconstitution
- For reconstitution add 200 μL of sterile water.
- Storage
- -20 °C
- Storage Comment
- store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
-
- Target
- HSP18.5 (HSP18.5-C) (18.5 kDa Class I Heat Shock Protein (HSP18.5-C))
- Alternative Name
- HSP18.5
- Background
- HSP18.5 is a small heat shock protein localized in the cytoplasm. The protein belongs to HSP20 family. Synonymes:AtHsp18.5, 18.5 kDa heat shock protein
- Molecular Weight
- 18.5 kDa
-
You are here:
