PAX6 antibody (N-Term) (CF®488A)
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- Target See all PAX6 Antibodies
- PAX6 (Paired Box 6 (PAX6))
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Binding Specificity
- AA 1-300, N-Term
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This PAX6 antibody is conjugated to CF®488A
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Application
- Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Purpose
- Mouse Monoclonal anti-PAX6 (PAX6/1166), CF488A Conjugate
- Characteristics
- Pax genes contain paired domains with strong homology to genes in Drosophila, which are involved in programming early development. Lesions in the Pax-6 gene account for most cases of aniridia, a congenital malformation of the eye, chiefly characterized by iris hypoplasia, which can cause blindness. Pax-6 is involved in other anterior segment malformations besides aniridia, such as Peters anomaly, a major error in the embryonic development of the eye with corneal clouding with variable iridolenticulocorneal adhesions. The Pax-6 gene encodes a transcriptional regulator that recognizes target genes through its paired-type DNA-binding domain. The paired domain is composed of two distinct DNA-binding subdomains, the amino-terminal subdomain and the carboxy-terminal subdomain, which bind respective consensus DNA sequences. The human Pax-6 gene produces two alternatively spliced isoforms that have the distinct structure of the paired domain. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- Immunogen
- Recombinant fragment (N-terminus, aa 1-300) of human PAX6 protein
- Clone
- PAX6-1166
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product PAX6 Primary Antibody
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- Application Notes
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Immunohistology formalin-fixed 0.5-1 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris buffer with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes
- Immunofluorescence 0.5-1 μg/mL
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Optimal dilution for a specific application should be determined by user
- Comment
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Pancreas, Cerebellum or Stomach
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 100 μg/mL
- Buffer
- PBS/0.1 % BSA/0.05 % azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Protect from light
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- Target
- PAX6 (Paired Box 6 (PAX6))
- Alternative Name
- PAX6 (PAX6 Products)
- Synonyms
- pax6 antibody, an2 antibody, mgda antibody, wagr antibody, pax-6 antibody, pax6a antibody, xpax6 antibody, XLPAX6 antibody, MGC52531 antibody, PAX-6 antibody, AN antibody, AN2 antibody, D11S812E antibody, MGDA antibody, WAGR antibody, Pax-6 antibody, pax6b antibody, 1500038E17Rik antibody, AEY11 antibody, Dey antibody, Gsfaey11 antibody, Sey antibody, PAXZF-A antibody, Pax6.1 antibody, cb280 antibody, fc20e07 antibody, pax-a antibody, pax[zf-a] antibody, paxzfa antibody, wu:fc20e07 antibody, zfpax-6a antibody, zfpax-6b antibody, paired box 6 antibody, paired box 6 L homeolog antibody, paired box 6 S homeolog antibody, paired box 6a antibody, PAX6 antibody, pax6 antibody, Pax6 antibody, pax6.L antibody, pax6.S antibody, pax6a antibody
- Background
- AN2, Aniridia type II protein, Oculorhombin, Paired box gene 6 (aniridia keratitis), Paired box homeotic gene 6, Paired box protein Pax-6, Sey, WAGR
- Molecular Weight
- 47 kDa
- Gene ID
- 5080, 611376
- UniProt
- P26367
- Pathways
- Peptide Hormone Metabolism, Carbohydrate Homeostasis
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