Retinoblastoma 1 antibody (Biotin)
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- Target See all Retinoblastoma 1 (RB1) Antibodies
- Retinoblastoma 1 (RB1)
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Reactivity
- Human, Mouse
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This Retinoblastoma 1 antibody is conjugated to Biotin
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)
- Purpose
- Mouse Monoclonal anti-Rb1 (RB1/1754), Biotin Conjugate
- Characteristics
- This antibody recognizes a 105 kDa phosphoprotein, identified as retinoblastoma (Rb) gene product. It shows no cross reaction with p107 or p130. It reacts with the hyper-phosphorylated as well as the un (under) phosphorylated form of the Rb protein. Retinoblastoma gene product plays a key role in cell cycle control. It has been identified as a tumor suppressor gene whose loss of its function leads to tumor development. It is widely expressed in a variety of human tissues including breast, esophageal, squamous cell and cervical carcinoma. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- Immunogen
- Recombinant human Rb1 protein
- Clone
- RB1-1754
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product RB1 Primary Antibody
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- Application Notes
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Immunohistology (formalin) 1-2 μg/mL
- Western blotting 0.5-1 μg/mL Immunoprecipitation 0.5-1 μg/500 μg protein lysate
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
- Optimal dilution for a specific application should be determined by user
- Comment
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HeLa, A431 or BT-20 cells. Breast or Colon carcinoma.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 100 μg/mL
- Buffer
- PBS/0.1 % BSA/0.05 % azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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- Target
- Retinoblastoma 1 (RB1)
- Alternative Name
- Rb1 (RB1 Products)
- Synonyms
- OSRC antibody, RB antibody, p105-Rb antibody, pRb antibody, pp110 antibody, Rb antibody, Rb-1 antibody, P105-RB antibody, PP110 antibody, RB11 antibody, zgc:154147 antibody, RB transcriptional corepressor 1 antibody, RB transcriptional corepressor like 1 antibody, retinoblastoma 1 antibody, RB1 antibody, RBL1 antibody, Rb1 antibody, rb1 antibody
- Background
- OSRC, Osteosarcoma, p105-Rb, PP105, pp110, pRb, Prepro retinoblastoma associated protein, RB1, Retinoblastoma 1, Retinoblastoma-associated protein
- Molecular Weight
- 105 kDa
- Gene ID
- 5925, 408528
- UniProt
- P06400
- Pathways
- Cell Division Cycle, Intracellular Steroid Hormone Receptor Signaling Pathway, Mitotic G1-G1/S Phases, DNA Replication, Maintenance of Protein Location, Synthesis of DNA, Autophagy
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