Neurofilament antibody

Catalog No. ABIN6188773

Product Details anti-Neurofilament Antibody

Target: Neurofilament

Reactivity: Human, Rat, Mouse, Pig, Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This Neurofilament antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunofluorescence (IF)

Purpose: Mouse Monoclonal anti-Neurofilament (H+L) (NF421 + NFL736), Purified, with BSA

Characteristics: This MAb reacts with a 200 kDa and 68 kDa protein, identified as heavy and light sub-units of neurofilaments (NF-H & NF-L). Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68 kDa (NF-L), 160 kDa (NF-M) and 200 kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilament. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Purification: Purified

Immunogen: Recombinant human neurofilament heavy sub-unit (RT-97) & light sub-unit (NR-4)

Clone: NF421 NFL-736

Isotype: IgG

Application Details

Application Notes: Immunohistology formalin-fixed 0.5-1 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 1:25-1:50
• Optimal dilution for a specific application should be determined by user

Comment:

Brain, Neuroblastoma

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 200 μg/mL

Buffer: PBS/0.05 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Target Details for Neurofilament

Target: Neurofilament

Abstract: Neurofilament Products

Molecular Weight: 200 kDa & 68 kDa

Gene ID: 4744, 198760

UniProt: P12036