GFAP antibody (CF®568)
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- Target See all GFAP Antibodies
- GFAP (Glial Fibrillary Acidic Protein (GFAP))
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Reactivity
- Human, Rat, Mouse, Pig, Cow, Chicken, Rabbit
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This GFAP antibody is conjugated to CF®568
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Application
- Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Purpose
- Mouse Monoclonal anti-GFAP (GA-5 + ASTRO/789), CF568 Conjugate
- Characteristics
- This MAb recognizes a protein of ~50 kDa which is identified as Glial Fibrillary Acidic Protein (GFAP). It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- Immunogen
- GFAP isolated from pig spinal cord (GA-5), Recombinant GFAP protein (ASTRO/789)
- Clone
- GA-5 ASTRO-789
- Isotype
- IgG
- Top Product
- Discover our top product GFAP Primary Antibody
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- Application Notes
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Immunohistology formalin-fixed 0.25-0.5 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Immunofluorescence 1-2 μg/mL
- Western blotting 0.5-1 μg/mL
- Optimal dilution for a specific application should be determined by user
- Comment
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Brain or Astrocytoma
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 100 μg/mL
- Buffer
- PBS/0.1 % BSA/0.05 % azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Protect from light
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- Target
- GFAP (Glial Fibrillary Acidic Protein (GFAP))
- Alternative Name
- GFAP (GFAP Products)
- Synonyms
- GFAP antibody, AI836096 antibody, cb345 antibody, etID36982.3 antibody, gfapl antibody, wu:fb34h11 antibody, wu:fk42c12 antibody, xx:af506734 antibody, zgc:110485 antibody, glial fibrillary acidic protein antibody, GFAP antibody, LOC100136168 antibody, gfap antibody, Gfap antibody
- Background
- Astrocyte or Intermediate Filament Protein, Glial Fibrillary Acidic Protein (GFAP)
- Molecular Weight
- ~50 kDa
- Gene ID
- 2670, 514227
- UniProt
- P14136
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