PODXL antibody (Extracellular Domain)
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- Target See all PODXL Antibodies
- PODXL (Podocalyxin-Like (PODXL))
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Binding Specificity
- Extracellular Domain
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Reactivity
- Human, Rat, Rabbit
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This PODXL antibody is un-conjugated
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Application
- Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Purpose
- Mouse Monoclonal anti-Podocalyxin (3D3), Purified, BSA-free
- Characteristics
- Podocalyxin is a member of the CD34 transmembrane sialomucin family. It is over-expressed on the podocyte foot projections and plays essential roles in kidney development and homeostasis, blood filtration and urine formation. It is also expressed on vascular endothelia, hematopoietic progenitors and a subset of neurons. Overexpression of podocalyxin may be linked to more aggressive tumor behavior. Podocalyxin antibody can identify podocytes in the urine (podocyturia) that may indicate glomerular disease, pre-eclampsia, and other kidney pathology. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- Purification
- Purified
- Immunogen
- A recombinant protein fragment containing the intracellular, transmembrane, and part of the extracellular domain of human podocalyxin
- Clone
- 3D3
- Isotype
- IgG1
- Top Product
- Discover our top product PODXL Primary Antibody
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- Application Notes
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Immunohistology formalin-fixed 0.5-1 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Immunofluorescence 1-2 μg/mL
- Western blotting 0.5-1 μg/mL
- Immunoprecipitation 1-2 μg/500 μg protein
- Optimal dilution for a specific application should be determined by user
- Comment
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HeLa, Raji, Jurkat cells, Angiosarcoma, Breast, Prostate, Liver, Pancreatic, Kidney
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- PBS (no BSA, no azide)
- Preservative
- Azide free
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- Target
- PODXL (Podocalyxin-Like (PODXL))
- Alternative Name
- Podocalyxin (PODXL Products)
- Synonyms
- Gp200 antibody, PC antibody, PCLP antibody, PCLP-1 antibody, PCB antibody, AW121214 antibody, Ly102 antibody, Pclp1 antibody, podocalyxin antibody, MEP21 antibody, PODXL antibody, Gp135 antibody, PCLP1 antibody, cPCLP1 antibody, podxl antibody, fc18d02 antibody, wu:fc18d02 antibody, Pc antibody, Pcb antibody, Podxl antibody, podocalyxin like antibody, pyruvate carboxylase antibody, podocalyxin-like antibody, PODXL antibody, PC antibody, Podxl antibody, podxl antibody, Pcx antibody
- Background
- PODXL, PDXL, GCTM-2, Gp200, PCLP1, Pcx, Podocalyxin-like protein 1
- Molecular Weight
- 165-170 kDa
- Gene ID
- 5420, 732423
- UniProt
- O00592
- Pathways
- Tube Formation
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