Myosin (Smooth Muscle Heavy Chain) antibody (CF®405S)

Catalog No. ABIN6180074

Product Details anti-Myosin (Smooth Muscle Heavy Chain) Antibody (CF®405S)

Target: Myosin (Smooth Muscle Heavy Chain)

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Myosin (Smooth Muscle Heavy Chain) antibody is conjugated to CF®405S

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Purpose: Mouse Monoclonal anti-Myosin, Smooth Muscle Heavy chain (MYH11/923 + SMMS-1), CF405S Conjugate

Characteristics: Smooth muscle myosin heavy chain (SM-MHC) is a cytoplasmic structural protein, which is a major component of the contractile apparatus in smooth muscle cells. Expression of smooth muscle myosin is developmentally regulated, appearing early in smooth muscle development, and is specific for smooth muscle development. Two isoforms of smooth muscle myosin heavy chain have been identified, designated MHC-1 and MHC-2. The antibody may be useful for the study of breast tumors as the presence of an intact layer of myoepithelial cells is an important feature, which may distinguish benign breast lesions and carcinoma in situ from invasive tumors. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human MYH11 protein (MYH11/923), Human uterus extract (SMMS-1)

Clone: MYH11-923 SMMS-1

Isotype: IgG

Application Details

Application Notes: Immunohistology formalin-fixed 0.5-1 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Predicted to show broad species reactivity
• Optimal dilution for a specific application should be determined by user

Comment:

Uterus or normal breast

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for Myosin (Smooth Muscle Heavy Chain)

Target: Myosin (Smooth Muscle Heavy Chain)

Alternative Name: Myosin, Smooth Muscle Heavy chain (Myosin (Smooth Muscle Heavy Chain) Products)

Background: AAT4, Myosin heavy chain 11 (MYH11), Myosin heavy chain 11 smooth muscle, Smooth muscle myosin heavy chain 11, Myosin-11, SM1, SM2, SMHC, SMMHC

Molecular Weight: 205 kDa (MHC-1) and 200 kDa (MHC-2)

Gene ID: 4629, 460109

UniProt: P35749