NPY1R antibody (AA 356-382)

Catalog No. ABIN617932

Product Details anti-NPY1R Antibody

Target: NPY1R (Neuropeptide Y Receptor 1 (NPY1R))

Binding Specificity: AA 356-382

Reactivity: Human, Rat, Mouse, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NPY1R antibody is un-conjugated

Application: Immunofluorescence (IF), Immunoelectron Microscopy (IEM), Immunocytochemistry (ICC), Immunohistochemistry (IHC)

Cross-Reactivity: Guinea Pig, Human, Mouse (Murine), Rat (Rattus)

Characteristics: The Neuropeptide Y Y1 Receptor was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat cortex, arcuate and hippocampus using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilutions are 1/500 - 1/1000 in PBS - Bn/Av-HRP detection. The antibody was characterized by immunohistochemistry and Western blot. Western blot showed one immunoreactive band of 40 kD and a single high molecular weight band, presumably a precursor molecule. Preincubation of the antibody with an excess of the synthetic peptide blocked staining. Immunohistochemical staining of rat brain correlates well with Northern analysis, in situ hybridization and receptor autoradiography. BlastP database sequence homology searches confirmed that this sequence is unique to rat, mouse and human NPY Y1 receptors.

Purification: Affinity Purified

Immunogen: Rat NPY Y1 receptor (356-382)

Isotype: IgG

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Contains ≤ 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: After reconstitution, use immediately or refrigerate at 2 - 8 °C up to 2 days. For long-term storage aliquot antibody and freeze at -15 °C or lower. Avoid repeated freeze/thaw cycles.

References for anti-NPY1R antibody (ABIN617932)

Dubois, Ramamoorthy, Whim, Liu: "Activation of NPY type 5 receptors induces a long-lasting increase in spontaneous GABA release from cerebellar inhibitory interneurons." in: Journal of neurophysiology, Vol. 107, Issue 6, pp. 1655-65, (2012) (PubMed).

Klenke, Constantin, Wray: "Neuropeptide Y directly inhibits neuronal activity in a subpopulation of gonadotropin-releasing hormone-1 neurons via Y1 receptors." in: Endocrinology, Vol. 151, Issue 6, pp. 2736-46, (2010) (PubMed).

Killough, Lundy, Irwin: "Dental pulp fibroblasts express neuropeptide Y Y1 receptor but not neuropeptide Y." in: International endodontic journal, Vol. 43, Issue 10, pp. 835-42, (2010) (PubMed).

Dailey, Bartness: "Arcuate nucleus destruction does not block food deprivation-induced increases in food foraging and hoarding." in: Brain research, Vol. 1323, pp. 94-108, (2010) (PubMed).

El Karim, Lamey, Linden, Lundy: "Neuropeptide Y Y1 receptor in human dental pulp cells of noncarious and carious teeth." in: International endodontic journal, Vol. 41, Issue 10, pp. 850-5, (2008) (PubMed).

Gibbs, Diogenes, Hargreaves: "Neuropeptide Y modulates effects of bradykinin and prostaglandin E2 on trigeminal nociceptors via activation of the Y1 and Y2 receptors." in: British journal of pharmacology, Vol. 150, Issue 1, pp. 72-9, (2007) (PubMed).

Zhang, Ossipov, Zhang, Lai, Porreca: "Nerve injury-induced tactile allodynia is present in the absence of FOS labeling in retrogradely labeled post-synaptic dorsal column neurons." in: Pain, Vol. 129, Issue 1-2, pp. 143-54, (2007) (PubMed).

Dimitrov, DeJoseph, Brownfield, Urban: "Involvement of neuropeptide Y Y1 receptors in the regulation of neuroendocrine corticotropin-releasing hormone neuronal activity." in: Endocrinology, Vol. 148, Issue 8, pp. 3666-73, (2007) (PubMed).

Urban, Leitermann, DeJoseph, Somponpun, Wolak, Sladek: "Influence of dehydration on the expression of neuropeptide Y Y1 receptors in hypothalamic magnocellular neurons." in: Endocrinology, Vol. 147, Issue 9, pp. 4122-31, (2006) (PubMed).

Li, Ritter: "Functional expression of neuropeptide Y receptors in human neuroblastoma cells." in: Regulatory peptides, Vol. 129, Issue 1-3, pp. 119-24, (2005) (PubMed).

Mills, Sohn, Micevych: "Estrogen-induced mu-opioid receptor internalization in the medial preoptic nucleus is mediated via neuropeptide Y-Y1 receptor activation in the arcuate nucleus of female rats." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 24, Issue 4, pp. 947-55, (2004) (PubMed).

Hiruma, Saito, Kusakabe, Takenaka, Kawakami: "Neuropeptide Y inhibits axonal transport of particles in neurites of cultured adult mouse dorsal root ganglion cells." in: The Journal of physiology, Vol. 543, Issue Pt 1, pp. 85-97, (2002) (PubMed).

Target Details for NPY1R

Target: NPY1R (Neuropeptide Y Receptor 1 (NPY1R))

Alternative Name: Neuropeptide Y receptor 1 (NPY1R Products)

Background: Other Names:
Neuropeptide Y receptor 1,NPY receptor 1, NPYR, NPYY1,NPY1-R,Neuropeptide Y receptor type 1, FC5, Npy1r,neuropeptide Y1 receptor, NPY1

Pathways: Feeding Behaviour