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Recombinant Keratin Basic antibody (Biotin)

Reactivity: Human WB, IF, FACS, IHC Host: Rabbit Monoclonal KRTH-1576R Biotin Recombinant Antibody
Catalog No. ABIN6178811
  • Target See all Keratin Basic Antibodies
    Keratin Basic
    Antibody Type
    Recombinant Antibody
    Reactivity
    • 20
    • 12
    • 9
    • 7
    • 7
    • 6
    • 4
    • 2
    • 2
    • 1
    • 1
    Human
    Host
    • 13
    • 8
    Rabbit
    Clonality
    • 21
    Monoclonal
    Conjugate
    • 14
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Biotin
    Application
    • 19
    • 17
    • 15
    • 12
    • 9
    • 4
    • 3
    • 3
    • 2
    • 1
    Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (IHC)
    Purpose
    Recombinant Rabbit Monoclonal anti-Cytokeratin, Basic (KRTH/1576R), Biotin Conjugate
    Characteristics
    This MAb recognizes basic (Type II or HMW) cytokeratins, which include 67 kDa (CK1), 64 kDa (CK3), 59 kDa (CK4), 58 kDa (CK5), 56 kDa (CK6), 52 kDa (CK8). Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50 , 48, 46, 45, and 40 kDa. MAb AE3 recognizes the 65-67, 64, 59, 58, 56, and 52 kDa keratins of basic subfamily. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
    Immunogen
    Recombinant full-length human KRT76 protein
    Clone
    KRTH-1576R
    Isotype
    IgG kappa
    Top Product
    Discover our top product Primary Antibody
  • Application Notes
    Immunohistology (formalin) 0.25-0.5 μg/mL
    • Immunofluorescence 1-2 μg/mL Western blotting 0.5-1 μg/mL
    • Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
    • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
    • Optimal dilution for a specific application should be determined by user
    Comment

    Epithelial cells, Skin or Adenocarcinomas

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    100 μg/mL
    Buffer
    PBS/0.1 % BSA/0.05 % azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Target
    Keratin Basic
    Alternative Name
    Cytokeratin, Basic (Keratin Basic Products)
    Background
    KRT2B, KRT2P, HUMCYT2A, Keratin, type II Cytoskeletal 2 oral, K76, Keratin 2p (K2P), Keratin-76, Cytokeratin-2P (CK-2P, Type-II Keratin Kb9
    Molecular Weight
    52-67 kDa
    Gene ID
    51350, 654392
    UniProt
    Q01546
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