Keratin 5/8 antibody (CF®568)
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- Target See all Keratin 5/8 (KRT5/8) products
- Keratin 5/8 (KRT5/8)
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Reactivity
- Human, Mouse, Pig, Rat, Dog, Hamster, Cow, Sheep, Ferret
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This Keratin 5/8 antibody is conjugated to CF®568
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Application
- Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Purpose
- Mouse Monoclonal anti-Cytokeratin 5/8 (C-50), CF568 Conjugate
- Characteristics
- This antibody recognizes keratin 5 (58 kDa) and keratin 8 (52.5 kDa). Simple epithelia express cytokeratin 8 in combination with 18. On the other hand, basal cells of stratified epithelia express cytokeratin 5 paired with 14. This antibody therefore, reacts with a wide range of epithelia and their carcinomas. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- Immunogen
- Cytoskeletal preparation from HeLa cells
- Clone
- C-50
- Isotype
- IgG1 kappa
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- Application Notes
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Immunohistology formalin-fixed 0.5-1.0 μg/mL
- For staining of formalin-fixed tissues, digest sections with trypsin at 1 mg/mL PBS, 15 min at RT
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Immunofluorescence 0.5-1.0 μg/mL
- Western blotting 0.5-1.0 μg/mL
- Does not react with chicken , or Xenopus
- Optimal dilution for a specific application should be determined by user
- Comment
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HepG2 or A431 cells. Skin or Squamous cell carcinoma
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 100 μg/mL
- Buffer
- PBS/0.1 % BSA/0.05 % azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Protect from light
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- Target
- Keratin 5/8 (KRT5/8)
- Alternative Name
- Cytokeratin 5/8 (KRT5/8 Products)
- Molecular Weight
- 58 kDa (CK5), 52.5 kDa (CK8)
- Gene ID
- 3852, 3856
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