Recombinant MLANA antibody
-
- Target See all MLANA Antibodies
- MLANA (Melan A (MLANA))
- Antibody Type
- Recombinant Antibody
-
Reactivity
- Human, Mouse, Rat, Dog
-
Host
- Mouse
-
Clonality
- Monoclonal
-
Conjugate
- This MLANA antibody is un-conjugated
-
Application
- Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunostaining (ISt)
- Purpose
- Recombinant Mouse Monoclonal anti-MART-1 / Melan-A / MLANA (rMLANA/788), Purified, with BSA
- Characteristics
- This antibody recognizes a protein doublet of 20-22 kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This MAb labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- Purification
- Purified
- Immunogen
- Recombinant full-length human MLANA protein
- Clone
- RMLANA-788
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product MLANA Primary Antibody
-
-
- Application Notes
-
Immunohistology (formalin) 0.5-1 μg/mL
- Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM citrate buffer pH 6.0for 10-20 min followed by cooling at RT for 20 min
- Immunofluorescence 0.5-1 μg/mL
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Western blotting 0.5-1 μg/mL
- Optimal dilution for a specific application should be determined by user
- Comment
-
SK-MEL-13 and SK-MEL-19 Melanoma cell lines, Melanomas
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 200 μg/mL
- Buffer
- PBS/0.05 % BSA/0.05 % azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
- Target
- MLANA (Melan A (MLANA))
- Alternative Name
- MART-1 / Melan-A / MLANA (MLANA Products)
- Synonyms
- A930034P04Rik antibody, Mart1 antibody, MART-1 antibody, MART1 antibody, melan-A antibody, Mlana antibody, MLANA antibody
- Background
- Antigen LB39-AA, Antigen SK29-AA, Melanoma antigen recognized by T-cells 1, MLAN-A, MLANA
- Molecular Weight
- 20-22 kDa (doublet)
- Gene ID
- 2315, 154069
- UniProt
- Q16655
-