FLI1 antibody (AA 107-261)
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- Target See all FLI1 Antibodies
- FLI1 (Friend Leukemia Virus Integration 1 (FLI1))
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Binding Specificity
- AA 107-261
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This FLI1 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)
- Purpose
- Mouse Monoclonal anti-FLI1 (FLI1/1312), Purified, with BSA
- Characteristics
- This antibody recognizes a protein of 51 kDa, which is identified as FLI1. This protein, a member of the ETS family of DNA binding transcription factors, is involved in cellular proliferation and tumorigenesis. Ets-1 is the prototype member of a family of genes identified on the basis of homology to the v-Ets oncogene isolated from the E26 erythroblastosis virus.Members of the Ets gene family share a highly conserved carboxy-terminal domain containing a sequence related to the SV40 large T antigen nuclear localization signal sequence. Approximately 90 % of Ewing s Sarcoma (EWS)/Primitive Neuroectodermal Tumors (PNET) have a specific translocation, t(11,22)(q24,q12), which results in fusion of EWS to Fli-1, and production of an EWS-Fli-1 fusion protein. Among normal tissues only endothelial cells and small lymphocytes express Fli-1. This protein is expressed in majority of vascular tumors including angiosarcomas, hemangioendotheliomas, hemangiomas, and Kaposi s Sarcomas. High sensitivity and specificity of Fli-1 equals to or exceeds that of the established vascular markers like CD31, CD34, and Factor VIII. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- Purification
- Purified
- Immunogen
- Recombinant fragment (aa 107-261) of human FLI1 protein (exact sequence is proprietary)
- Clone
- FLI1-1312
- Isotype
- IgG
- Top Product
- Discover our top product FLI1 Primary Antibody
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- Application Notes
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Western blotting 1-2 μg/mL
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Immunofluorescence 1-2 μg/mL
- Optimal dilution for a specific application should be determined by user
- Comment
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THP1 and Raji cells. Tonsil, Ewing s sarcoma (EWS) and Angiosarcoma
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 200 μg/mL
- Buffer
- PBS/0.05 % BSA/0.05 % azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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- Target
- FLI1 (Friend Leukemia Virus Integration 1 (FLI1))
- Alternative Name
- FLI1 (FLI1 Products)
- Synonyms
- EWSR2 antibody, SIC-1 antibody, FLI1 antibody, fli antibody, Xfli antibody, fli1 antibody, Xfli1 antibody, fli-1 antibody, Xfli-1 antibody, MGC121593 antibody, Fli-1 antibody, Sic1 antibody, cb855 antibody, wu:fc45b11 antibody, xlfli antibody, Fli-1 proto-oncogene, ETS transcription factor antibody, Fli-1 proto-oncogene, ETS transcription factor S homeolog antibody, Friend leukemia integration 1 antibody, Fli-1 proto-oncogene, ETS transcription factor a antibody, Fli-1 proto-oncogene, ETS transcription factor L homeolog antibody, FLI1 antibody, Fli1 antibody, fli1.S antibody, fli1 antibody, fli1a antibody, fli1.L antibody
- Molecular Weight
- 51 kDa
- Gene ID
- 2313, 504281
- UniProt
- Q01543
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