CPK antibody

Catalog No. ABIN6169697

Product Details anti-CPK Antibody

Target: CPK (Creatine Phosphokinase (CPK))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CPK antibody is un-conjugated

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Purpose: Mouse Monoclonal anti-Creatine Phosphokinase (2ba6), Purified, with BSA

Characteristics: Creatine kinases (CK) are a large family of isoenzymes that regulate levels of ATP in subcellular compartments, where they provide ATP at sites of fluctuating energy demand by the transfer of phosphates between creatine and adenine nucleotides. CKs provide the energy of phosphate hydrolysis necessary to drive the normal function of many cellular systems. In cells, the cytosolic CK enzymes consist of two subunits, which can be either B (brain type) or M (muscle type). There are three different isoenzymes: CKMM, CKBB and CKMB. This MAb recognizes the CKBB isoenzyme and does not react with the B subunit in CKMB. It shows minimal reactivity with other human serum proteins Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Purification: Purified

Immunogen: Human CKBB protein

Clone: 2ba6

Isotype: IgG1 kappa

Application Details

Application Notes: Immunohistology Frozen Only 0.5-1.0 μg/mL

• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Predicted to show broad species reactivity
• Optimal dilution for a specific application should be determined by user

Comment:

Cerebellum

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 200 μg/mL

Buffer: PBS/0.05 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Target Details for CPK

Target: CPK (Creatine Phosphokinase (CPK))

Alternative Name: Creatine Phosphokinase (CPK Products)

Molecular Weight: 43 kDa (Monomer), 86 kDa (Dimer)

Gene ID: 1152, 173724

UniProt: P12277