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KRT1 antibody (CF®405S)

KRT1 Reactivity: Human, Rat, Mouse WB, IF, FACS, IHC (f) Host: Mouse Monoclonal 34BE12 CF®405S
Catalog No. ABIN6161927
  • Target See all KRT1 Antibodies
    KRT1 (Cytokeratin HMW (KRT1))
    Reactivity
    • 27
    • 27
    • 18
    • 10
    • 10
    • 10
    • 10
    • 9
    • 1
    Human, Rat, Mouse
    Host
    • 27
    Mouse
    Clonality
    • 27
    Monoclonal
    Conjugate
    • 7
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    This KRT1 antibody is conjugated to CF®405S
    Application
    • 26
    • 26
    • 17
    • 15
    • 9
    • 9
    • 2
    • 2
    Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
    Purpose
    Mouse Monoclonal anti-Cytokeratin, HMW (34BE12), CF405S Conjugate
    Characteristics
    This antibody recognizes CK1, CK5, CK10 and CK14. In normal epithelia, it stains stratified epithelia, myoepithelial cells and basal cells in the prostate gland and bronchi. This MAb shows no reactivity with hepatocytes, pancreatic acinar cells, proximal renal tubules, or endometrial glands, there is no reactivity with cells derived from simple epithelia. Mesenchymal tumors, lymphomas, melanomas, neural tumors, and neuroendocrine tumors are negative with this antibody. It stains myoepithelial cells and has been shown to be useful in distinguishing prostate adenocarcinoma from benign prostate. This antibody has also been useful in separating benign from malignant intraductal breast proliferations. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
    Immunogen
    Solubilized keratin extract from human stratum corneum
    Clone
    34BE12
    Isotype
    IgG1 kappa
    Top Product
    Discover our top product KRT1 Primary Antibody
  • Application Notes
    Immunohistology formalin-fixed 0.25-0.5 μg/mL
    • Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
    • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
    • Immunofluorescence 0.5-1.0 μg/mL
    • Western blotting 0.5-1.0 μg/mL
    • Optimal dilution for a specific application should be determined by user
    Comment

    PC12 cells, Skin, Prostate carcinoma

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    100 μg/mL
    Buffer
    PBS/0.1 % BSA/0.05 % azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Protect from light
  • Target
    KRT1 (Cytokeratin HMW (KRT1))
    Alternative Name
    Cytokeratin, HMW (KRT1 Products)
    Synonyms
    CK1 antibody, EHK antibody, EHK1 antibody, EPPK antibody, K1 antibody, KRT1A antibody, NEPPK antibody, keratin 1 antibody, KRT1 antibody
    Background
    KRT2B, KRT2P, HUMCYT2A, Keratin, type II Cytoskeletal 2 oral, K76, Keratin 2p (K2P), Keratin-76, Cytokeratin-2P (CK-2P, Type-II Keratin Kb9
    Molecular Weight
    50-67 kDa
    Gene ID
    51350, 654392
    UniProt
    Q01546
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