Keratin 10/13 antibody (Biotin)

Catalog No. ABIN6161006

Product Details

Target: Keratin 10/13

Reactivity: Human, Cat

Host: Mouse

Clonality: Monoclonal

Conjugate: Biotin

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF)

Purpose: Mouse Monoclonal anti-Cytokeratin 10/13 (DE-K13), Biotin Conjugate

Characteristics: This antibody recognizes cytokeratin 10 (56.5 kDa) and cytokeratin 13 (53 kDa) in Western blotting. It recognizes only cytokeratin 13 in formalin-fixed, paraffin-embedded tissue sections. It does not react with cytokeratin 10 positive, cytokeratin 13 negative epithelia such as epidermis. However, on frozen sections this MAb serves as differentiation-related marker of all stratified epithelia, it stains all suprabasal cells in both cornifying and non-cornifying stratified epithelia and more differentiated cells of squamous carcinomas. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Cytoskeletal preparation extracted from human ectocervical epithelium

Clone: DE-K13

Isotype: IgG2a kappa

Application Details

Application Notes: Immunofluorescence 0.5-1 μg/mL

• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Western blotting 0.25-0.5 μg/mL

Comment:

A431, HeLa, MCF7 cells. Tonsil, esophageal or bladder carcinoma.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Target Details

Target: Keratin 10/13

Alternative Name: Cytokeratin 10/13

Background: BCIE, BIE, EHK, Keratin Type I Cytoskeletal 10, KRT10, CK13, K13, keratin type I cytoskeletal 13, Keratin-13, Krt1-13, KRT1

Molecular Weight: 56.5 kDa (CK10) & 53 kDa (CK13)

Gene ID: 3858, 99936

UniProt: P13645