CD6 antibody
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- Target See all CD6 Antibodies
- CD6
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This CD6 antibody is un-conjugated
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Application
- Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Purpose
- Mouse Monoclonal anti-CD6 (C6/372 + 3F7B5), Purified, with BSA
- Characteristics
- CD6 is a type I transmembrane glycoprotein that contains a 24-amino acid signal sequence, three extracellular ?scavenger receptor cysteine-rich? (SRCR) domains, a membrane-spanning domain and a 44-amino acid cytoplasmic domain. The CD6 glycoprotein is tyrosine phosphorylated during TCR-mediated T cell activation. CD6 shows significant homology to CD5. CD6 is present on mature thymocytes, peripheral T cells and a subset of B cells. Antibodies to CD6 are used to deplete T cells from bone marrow transplants to prevent graft versus host disease. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- Purification
- Purified
- Immunogen
- Human recombinant CD6 protein (C6/372), Human rheumatoid synovial T cell line ST-1 (3F7B5)
- Clone
- C6-372 3F7B5
- Isotype
- IgG
- Top Product
- Discover our top product CD6 Primary Antibody
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- Application Notes
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Immunohistology formalin-fixed 0.5-1 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
- Immunofluorescence 0.5-1 μg/mL
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Optimal dilution for a specific application should be determined by user
- Comment
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CCRF-CEM, Jurkat cells, Tonsil
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 200 μg/mL
- Buffer
- PBS/0.05 % BSA/0.05 % azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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- Target
- CD6
- Alternative Name
- CD6 (CD6 Products)
- Synonyms
- TP120 antibody, OX52 antibody, CD6 antigen antibody, CD6 molecule antibody, Cd6 molecule antibody, Cd6 antibody, CD6 antibody
- Background
- T12, TP120
- Molecular Weight
- 90-130 kDa
- Gene ID
- 923, 744366
- UniProt
- P30203
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