Melanoma Marker antibody

Catalog No. ABIN6157176

Product Details anti-Melanoma Marker Antibody

Target: Melanoma Marker

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Melanoma Marker antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunofluorescence (IF)

Purpose: Mouse Monoclonal anti-Melanoma Marker (KBA.62), Purified, BSA-free

Characteristics: KBA.62 is a novel anti-melanoma antibody. It reacts positively against melanocytic tumors but not other tumors, thus demonstrating specificity and sensitivity. Moreover, it reacts positively against junctional nevus cells but not intradermal nevi, and against fetal melanocytes but not normal adult melanocytes. KBA.62 antibody is useful in identifying malignant melanomas. Metastatic amelanotic melanoma can often be confused with a variety of poorly differentiated carcinomas, large cell lymphomas, sarcomas, spindle cell carcinomas and various types of mesenchymal neoplasms. A keratin-negative, vimentin-rich neoplasm that immuno-reacts with antibody to S-100 protein and with KBA.62 antibody is, with rare exception, a melanoma. Anti-KBA.62 is a useful additional marker for melanoma, specifically in desmoplastic/spindle cell cases and in the context of micro-metastasis in sentinel lymph node. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Purification: Purified

Immunogen: Human KAL cells derived from lymph node metastasis of malignant melanoma

Clone: KBA-62

Isotype: IgG1 kappa

Application Details

Application Notes: Immunohistology (formalin)

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Immunofluorescence 0.5-1 μg/mL
• Optimal dilution for a specific application should be determined by user

Comment:

SK-MEL-13 and SK-MEL-19 Melanoma cell lines, Melanomas

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS (no BSA, no azide)

Preservative: Azide free

Target Details for Melanoma Marker

Target: Melanoma Marker

Abstract: Melanoma Marker Products

Background: Human Melanoma Associated Antigen, KBA.62

Molecular Weight: Multiple (140, 135 and 128 kDa and two weak bands of 88 and 73 kDa)