Melanoma Marker antibody (CF®594)

Catalog No. ABIN6157125

Product Details anti-Melanoma Marker Antibody (CF®594)

Target: Melanoma Marker

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Melanoma Marker antibody is conjugated to CF®594

Application: Immunostaining (ISt), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Purpose: Mouse Monoclonal anti-Melanoma Marker (HMB45 + M2-7C10 + M2-9E3 + T311), CF594 Conjugate

Characteristics: This antibody cocktail recognizes three melanoma-specific proteins, which include MART-1, Tyrosinase and gp100. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Tyrosinase is one of the targets for cytotoxic T-cell recognition in melanoma patients. Function of gp100 is not known but it is reported to be a useful marker for melanocytes and melanomas. This cocktail of three markers is designed for extremely sensitive labeling of formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant hMART-1 protein (M2-7C10, M2-9E3), Recombinant tyrosinase protein (T311), Extract of pigmented melanoma metastases from lymph nodes (HMB45)

Clone: HMB45 M2-7C10 M2-9E3 T311

Isotype: IgG1, IgG2a, IgG2b kappa

Application Details

Application Notes: Immunohistology (Formalin-fixed) 0.5-1.0 μg/mL

• Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Optimal dilution for a specific application should be determined by user

Comment:

SK-MEL-13 and SK-MEL-19 Melanoma cell lines, Melanomas

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for Melanoma Marker

Target: Melanoma Marker

Abstract: Melanoma Marker Products

Background: Melanoma antigen recognized by T-cells 1 (MART-1), MLAN-A, TYR, PMEL17

Molecular Weight: 20-22 kDa (doublet) (MART), 70-80 kDa (Tyrosinase), 90-100 kDa (gp100)