TOP1MT antibody (CF®594)
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- Target See all TOP1MT Antibodies
- TOP1MT (Topoisomerase (DNA) I, Mitochondrial (TOP1MT))
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This TOP1MT antibody is conjugated to CF®594
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Application
- Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Purpose
- Mouse Monoclonal anti-Topoisomerase-I, MT (TOP1MT/488), CF594 Conjugate
- Characteristics
- DNA topoisomerases are nuclear enzymes that regulate the topological structure of DNA in eukaryotic cells by transiently breaking and rejoining DNA strands. Due to their roles in DNA replication, recombination, and transcription, DNA topoisomerases have been identified as targets of numerous anticancer drugs. Mitochondrial Topo I (DNA topoisomerase I, mitochondrial) is a 601 amino acid protein that primarily acts to relieve DNA strain that may occur during duplication of mitochondrial DNA. As a type IB topoisomerase, mitochondrial Topo I requires a divalent metal, either, calcium or magnesium, as well as an alkaline pH for optimal activity. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- Immunogen
- Recombinant full-length human TOP1MT protein
- Clone
- TOP1MT-488
- Isotype
- IgG2b kappa
- Top Product
- Discover our top product TOP1MT Primary Antibody
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- Application Notes
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Immunohistology formalin-fixed 0.5-1 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
- Immunofluorescence 0.5-1 μg/mL
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Optimal dilution for a specific application should be determined by user
- Comment
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A431 cells. Heart, Skeletal muscle, brain or fetal liver.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 100 μg/mL
- Buffer
- PBS/0.1 % BSA/0.05 % azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Protect from light
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- Target
- TOP1MT (Topoisomerase (DNA) I, Mitochondrial (TOP1MT))
- Alternative Name
- Topoisomerase-I, MT (TOP1MT Products)
- Synonyms
- 2900052H09Rik antibody, TOP1 antibody, topoisomerase (DNA) I, mitochondrial antibody, DNA topoisomerase 1, mitochondrial antibody, DNA topoisomerase I mitochondrial antibody, TOP1MT antibody, Top1mt antibody
- Background
- DNA topoisomerase I, TOP1MT, Topoisomerase I mitochondrial, Type IB Topoisomerase
- Molecular Weight
- 70 kDa
- Gene ID
- 116447, 528574
- UniProt
- Q969P6
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