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HLA-DP beta antibody (CF®594)

HLA-DP beta Reactivity: Human FACS, IF, IHC (f) Host: Mouse Monoclonal BRA-FB6 CF®594
Catalog No. ABIN6153325
  • Target See all HLA-DP beta products
    HLA-DP beta (MHC Class II HLA-DP beta (HLA-DP beta))
    Reactivity
    Human
    Host
    • 17
    Mouse
    Clonality
    • 17
    Monoclonal
    Conjugate
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    This HLA-DP beta antibody is conjugated to CF®594
    Application
    Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
    Purpose
    Mouse Monoclonal anti-MHC-II (HLA-DP beta chain) (BRA-FB6), CF594 Conjugate
    Characteristics
    This antibody recognizes a non-polymorphic determinant of DP-MHC class II. MHC class II antigens are transmembrane glycoproteins of non-covalently linked i± (33-35 kDa) and i² (27-30 kDa) chains. It reportedly reacts with B- & non-T, non-B cell lines but not with T- and myeloid cell lines and leukemias. Differential expression of MHC class II antigens on fetal and adult lymphocytes, malignant B cells appears to reflect the stage of cell differentiation which may be useful in the study of lymphoproliferative disorders. Primary antibodies are available purified, or with a selection of fluorescent CF ® dyes and other labels. CF ® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF ®405S and CF ®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
    Immunogen
    Non-T, non-B human acute lymphoblastic leukemia REH6 cell line
    Clone
    BRA-FB6
    Isotype
    IgG2b kappa
  • Application Notes
    Immunohistology formalin-fixed 0.5-1 μg/mL
    • Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
    • Immunofluorescence 0.5-1 μg/mL
    • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
    • Optimal dilution for a specific application should be determined by user
    Comment

    Raji cells. Tonsil or lymph node

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    100 μg/mL
    Buffer
    PBS/0.1 % BSA/0.05 % azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Protect from light
  • Target
    HLA-DP beta (MHC Class II HLA-DP beta (HLA-DP beta))
    Alternative Name
    MHC-II (HLA-DP) (beta chain) (HLA-DP beta Products)
    Molecular Weight
    33-35 kDa
    Gene ID
    3115, 347270
    UniProt
    P04440
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