HLA-DP beta antibody (CF®594)

Catalog No. ABIN6153325

Product Details anti-HLA-DP beta Antibody (CF®594)

Target: HLA-DP beta (MHC Class II HLA-DP beta (HLA-DP beta))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This HLA-DP beta antibody is conjugated to CF®594

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Purpose: Mouse Monoclonal anti-MHC-II (HLA-DP beta chain) (BRA-FB6), CF594 Conjugate

Characteristics: This antibody recognizes a non-polymorphic determinant of DP-MHC class II. MHC class II antigens are transmembrane glycoproteins of non-covalently linked i± (33-35 kDa) and i² (27-30 kDa) chains. It reportedly reacts with B- & non-T, non-B cell lines but not with T- and myeloid cell lines and leukemias. Differential expression of MHC class II antigens on fetal and adult lymphocytes, malignant B cells appears to reflect the stage of cell differentiation which may be useful in the study of lymphoproliferative disorders. Primary antibodies are available purified, or with a selection of fluorescent CF ® dyes and other labels. CF ® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF ®405S and CF ®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Non-T, non-B human acute lymphoblastic leukemia REH6 cell line

Clone: BRA-FB6

Isotype: IgG2b kappa

Application Details

Application Notes: Immunohistology formalin-fixed 0.5-1 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Comment:

Raji cells. Tonsil or lymph node

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for HLA-DP beta

Target: HLA-DP beta (MHC Class II HLA-DP beta (HLA-DP beta))

Alternative Name: MHC-II (HLA-DP) (beta chain) (HLA-DP beta Products)

Molecular Weight: 33-35 kDa

Gene ID: 3115, 347270

UniProt: P04440