Blood Group Lewis B antibody (CF®594)

Catalog No. ABIN6153097

Product Details anti-Blood Group Lewis B Antibody (CF®594)

Target: Blood Group Lewis B

Reactivity: Human, Guinea Pig, Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This Blood Group Lewis B antibody is conjugated to CF®594

Application: Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunostaining (ISt)

Purpose: Mouse Monoclonal anti-Lewis-B (2-25LE), CF594 Conjugate

Characteristics: The Lewis histo-blood group system comprises a set of fucosylated glycosphingolipids that are synthesized by exocrine epithelial cells and circulate in body fluids. The glycosphingolipids function in embryogenesis, tissue differentiation, tumor metastasis, inflammation, and bacterial adhesion. They are secondarily absorbed to red blood cells giving rise to their Lewis phenotype. This gene is a member of the fucosyltransferase family, which catalyzes the addition of fucose to precursor polysaccharides in the last step of Lewis antigen biosynthesis. It encodes an enzyme with alpha(1,3)-fucosyltransferase and alpha(1,4)-fucosyltransferase activities. Lewis blood group antigens are carbohydrate moieties structurally integrated in mucous secretions. Lewis antigen system alterations have been described in gastric carcinoma and associated lesions. Anomalous expression of Lewis B antigen has been found in some non-secretory gastric carcinomas and colorectal cancers. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Mucin isolated from a human ovarian cyst fluid

Clone: 2-25LE

Isotype: IgG1 kappa

Application Details

Application Notes: Immunohistology formalin-fixed 0.5-1 μg/mL

• Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Immunofluorescence 0.5-1 μg/mL
• Optimal dilution for a specific application should be determined by user

Comment:

Colon

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details for Blood Group Lewis B

Target: Blood Group Lewis B

Alternative Name: Lewis-B (Blood Group Lewis B Products)

Synonyms: MUC5 antibody, TBM antibody, leB antibody, mucin 5AC, oligomeric mucus/gel-forming antibody, MUC5AC antibody

Molecular Weight: 45 kDa

Gene ID: 2525, 169238

UniProt: P21217