Recognizes AKT (AKT1/AKT2/AKT3). The sequence used to generate this antibody, has a high degree of similarity to regions found in AKT1, AKT2 and AKT3, and thus may cross react with all of these proteins.
Cross-Reactivity (Details)
Species reactivity (expected):Chicken. Species reactivity (tested):Human, Mouse and Rat.
Purification
Delipidation and Defibrination
Immunogen
Synthetic peptide -KLH conjugated corresponding to the C-terminus (460-480) of Human, Rat, Mouse and Chicken AKT proteins conjugated to KLH using maleimide.
AKT is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways. AKT Exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis and (ii) promotion of proliferation.Synonyms: AKT-2, AKT-3 Review 010, Akt-1, C-AKT, PKB gamma, PKBG, Protein kinase B, RAC-PK-alpha, RAC-PK-beta, RAC-PK-gamma