UBE2O antibody (AA 1073-1292)

Catalog No. ABIN6149817

Product Details anti-UBE2O Antibody

Target: UBE2O (Ubiquitin-Conjugating Enzyme E2O (UBE2O))

Binding Specificity: AA 1073-1292

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBE2O antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF)

Sequence: LVNEPYYNEA GFDSDRGLQE GYENSRCYNE MALIRVVQSM TQLVRRPPEV FEQEIRQHFS TGGWRLVNRI ESWLETHALL EKAQALPNGV PKASSSPEPP AVAELSDSGQ QEPEDGGPAP GEASQGSDSE GGAQGLASAS RDHTDQTSET APDASVPPSV KPKKRRKSYR SFLPEKSGYP DIGFPLFPLS KGFIKSIRGV LTQFRAALLE AGMPECTEDK

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Polyclonal Antibodies

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1073-1292 of human UBE2O (NP_071349.3).

Isotype: IgG

Application Details

Application Notes: WB,1:1000 - 1:4000,IF,1:50 - 1:200,IP,1:50 - 1:200

Comment:

HIGH QUALITY

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide,50 % glycerol, pH 7.3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for UBE2O

Target: UBE2O (Ubiquitin-Conjugating Enzyme E2O (UBE2O))

Alternative Name: UBE2O (UBE2O Products)

Synonyms: e2-230k antibody, E2-230K antibody, 9630022H21 antibody, B230113M03Rik antibody, mKIAA1734 antibody, RGD1310297 antibody, ubiquitin conjugating enzyme E2 O antibody, ubiquitin-conjugating enzyme E2O antibody, UBE2O antibody, ube2o antibody, Ube2o antibody

Background: E2/E3 hybrid ubiquitin-protein ligase that displays both E2 and E3 ligase activities and mediates monoubiquitination of target proteins. Negatively regulates TRAF6-mediated NF-kappa-B activation independently of its E2 activity. Acts as a positive regulator of BMP7 signaling by mediating monoubiquitination of SMAD6, thereby regulating adipogenesis. Mediates monoubiquitination at different sites of the nuclear localization signal (NLS of BAP1, leading to cytoplasmic retention of BAP1. Also able to monoubiquitinate the NLS of other chromatin-associated proteins, such as INO80 and CXXC1, affecting their subcellular location. Acts as a regulator of retrograde transport by assisting the TRIM27:MAGEL2 E3 ubiquitin ligase complex to mediate 'Lys-63'-linked ubiquitination of WASHC1, leading to promote endosomal F-actin assembly.,UBE2O,E2-230K,Cell Biology & Developmental Biology,Ubiquitin,Ubiquitin-Proteasome Signaling Pathway,UBE2O

Molecular Weight: 141 kDa

Gene ID: 63893

UniProt: Q9C0C9