UBE2F antibody (AA 1-185)

Catalog No. ABIN6149794

Product Details anti-UBE2F Antibody

Target: UBE2F (Ubiquitin-Conjugating Enzyme E2F (UBE2F))

Binding Specificity: AA 1-185

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBE2F antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Sequence: MLTLASKLKR DDGLKGSRTA ATASDSTRRV SVRDKLLVKE VAELEANLPC TCKVHFPDPN KLHCFQLTVT PDEGYYQGGK FQFETEVPDA YNMVPPKVKC LTKIWHPNIT ETGEICLSLL REHSIDGTGW APTRTLKDVV WGLNSLFTDL LNFDDPLNIE AAEHHLRDKE DFRNKVDDYI KRYAR

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Polyclonal Antibodies

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-185 of human UBE2F (NP_542409.1).

Isotype: IgG

Application Details

Application Notes: WB,1:500 - 1:2000,IHC,1:50 - 1:200,IF,1:50 - 1:200

Comment:

HIGH QUALITY

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide,50 % glycerol, pH 7.3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for UBE2F

Target: UBE2F (Ubiquitin-Conjugating Enzyme E2F (UBE2F))

Alternative Name: UBE2F (UBE2F Products)

Synonyms: NCE2 antibody, 2510010F15Rik antibody, AI851109 antibody, RGD1307608 antibody, zgc:77005 antibody, ubiquitin conjugating enzyme E2 F (putative) antibody, NEDD8-conjugating enzyme UBE2F antibody, ubiquitin-conjugating enzyme E2F (putative) antibody, ubiquitin conjugating enzyme E2F (putative) S homeolog antibody, UBE2F antibody, Tsp_01616 antibody, ube2f antibody, ube2f.S antibody, Ube2f antibody

Background: Ube2F is an E2 enzyme, which is part of the E1, E2, and E3 cascade responsible for conjugation of Nedd8 to protein substrates. Neddylation is initiated by activating Nedd8 by the Nedd8 activating enzyme APPBP1/UBA3. Cullin is a well-known substrate for Neddylation. When NEDD8 is covalently bonded to the CRL, it stimulates its E3 Ub ligase activity. A new approach to cancer therapy involves hindering the Nedd8 conjugation pathway through inhibition of the NAE1/UBA3 enzyme.,UBE2F,NCE2,Epigenetics & Nuclear Signaling,Cell Biology & Developmental Biology,Ubiquitin,Ubiquitin-Proteasome Signaling Pathway,UBE2F

Molecular Weight: 11 kDa/13 kDa/17 kDa/18 kDa/21 kDa/23 kDa

Gene ID: 140739

UniProt: Q969M7