PARP1 antibody (AA 81-390)

Catalog No. ABIN6145232

Product Details anti-PARP1 Antibody

Target: PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))

Binding Specificity: AA 81-390

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PARP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF)

Sequence: DQQKVKKTAE AGGVTGKGQD GIGSKAEKTL GDFAAEYAKS NRSTCKGCME KIEKGQVRLS KKMVDPEKPQ LGMIDRWYHP GCFVKNREEL GFRPEYSASQ LKGFSLLATE DKEALKKQLP GVKSEGKRKG DEVDGVDEVA KKKSKKEKDK DSKLEKALKA QNDLIWNIKD ELKKVCSTND LKELLIFNKQ QVPSGESAIL DRVADGMVFG ALLPCEECSG QLVFKSDAYY CTGDVTAWTK CMVKTQTPNR KEWVTPKEFR EISYLKKLKV KKQDRIFPPE TSASVAATPP PSTASAPAAV NSSASADKPL

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Polyclonal Antibodies

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 81-390 of human PARP1 (NP_001609.2).

Isotype: IgG

Application Details

Application Notes: WB,1:1000 - 1:3000,IHC,1:50 - 1:200,IF,1:50 - 1:200,IP,1:50 - 1:200

Comment:

HIGH QUALITY

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide,50 % glycerol, pH 7.3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

References for anti-PARP1 antibody (ABIN6145232)

Huang, Cui, Chen, Yang, Lian, Liu, Su, Liu, Wang, Hu, Ouyang, Li, Lu: "NVP-BEZ235 synergizes cisplatin sensitivity in osteosarcoma." in: Oncotarget, Vol. 9, Issue 12, pp. 10483-10496, (2018) (PubMed).

Knowlton, Fernández de Castro, Ashbrook, Gestaut, Zamora, Bauer, Forrest, Frydman, Risco, Dermody: "The TRiC chaperonin controls reovirus replication through outer-capsid folding." in: Nature microbiology, Vol. 3, Issue 4, pp. 481-493, (2018) (PubMed).

Target Details for PARP1

Target: PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))

Alternative Name: PARP1 (PARP1 Products)

Background: This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes.,ADPRT,ADPRT 1,ADPRT1,ARTD1,PARP,PARP-1,PPOL,pADPRT-1,PARP1,Cleaved PARP,PARP1,Epigenetics & Nuclear Signaling,Chromatin Modifying Enzymes,other,DNA Damage & Repair,RNA Binding,Signal Transduction,Cell Biology & Developmental Biology,Cell Cycle,Centrosome,Death Receptor Signaling Pathway,Immunology & Inflammation,NF-kB Signaling Pathway,PARP1

Molecular Weight: 113 kDa

Gene ID: 142

UniProt: P09874

Pathways: Apoptosis, Caspase Cascade in Apoptosis, DNA Damage Repair, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location