METTL14 antibody (AA 1-210)

Catalog No. ABIN6143759

Product Details anti-METTL14 Antibody

Target: METTL14 (Methyltransferase Like 14 (METTL14))

Binding Specificity: AA 1-210

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This METTL14 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunofluorescence (IF)

Sequence: MDSRLQEIRE RQKLRRQLLA QQLGAESADS IGAVLNSKDE QREIAETRET CRASYDTSAP NAKRKYLDEG ETDEDKMEEY KDELEMQQDE ENLPYEEEIY KDSSTFLKGT QSLNPHNDYC QHFVDTGHRP QNFIRDVGLA DRFEEYPKLR ELIRLKDELI AKSNTPPMYL QADIEAFDIR ELTPKFDVIL LEPPLEEYYR ETGITANEKC

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Polyclonal Antibodies

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-210 of human METTL14 (NP_066012.1).

Isotype: IgG

Application Details

Application Notes: WB,1:500 - 1:2000,IHC,1:50 - 1:200,IF,1:50 - 1:200,IP,1:50 - 1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide,50 % glycerol, pH 7.3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

References for anti-METTL14 antibody (ABIN6143759)

Xiang, Yang, Liu, Wu, Chen, Yang: "N6-Methyladenosines Modulate A-to-I RNA Editing." in: Molecular cell, Vol. 69, Issue 1, pp. 126-135.e6, (2018) (PubMed).

Xiang, Laurent, Hsu, Nachtergaele, Lu, Sheng, Xu, Chen, Ouyang, Wang, Ling, Hsu, Zou, Jambhekar, He, Shi: "RNA m6A methylation regulates the ultraviolet-induced DNA damage response." in: Nature, Vol. 543, Issue 7646, pp. 573-576, (2017) (PubMed).

Yoon, Ringeling, Vissers, Jacob, Pokrass, Jimenez-Cyrus, Su, Kim, Zhu, Zheng, Kim, Wang, Doré, Jin, Regot, Zhuang, Canzar, He, Ming, Song: "Temporal Control of Mammalian Cortical Neurogenesis by m6A Methylation." in: Cell, Vol. 171, Issue 4, pp. 877-889.e17, (2017) (PubMed).

Target Details for METTL14

Target: METTL14 (Methyltransferase Like 14 (METTL14))

Alternative Name: METTL14 (METTL14 Products)

Synonyms: RGD1304822 antibody, G430022H21Rik antibody, mKIAA1627 antibody, zgc:77296 antibody, methyltransferase like 14 antibody, methyltransferase like 14 L homeolog antibody, Mettl14 antibody, mettl14.L antibody, METTL14 antibody, mettl14 antibody

Background: The METTL3-METTL14 heterodimer forms a N6-methyltransferase complex that methylates adenosine residues at the N(6 position of some mRNAs and regulates the circadian clock, differentiation of embryonic stem cells and cortical neurogenesis. In the heterodimer formed with METTL3, METTL14 constitutes the RNA-binding scaffold that recognizes the substrate rather than the catalytic core. N6-methyladenosine (m6A, which takes place at the 5'-[AG]GAC-3' consensus sites of some mRNAs, plays a role in mRNA stability and processing. M6A acts as a key regulator of mRNA stability by promoting mRNA destabilization and degradation (By similarity. In embryonic stem cells (ESCs, m6A methylation of mRNAs encoding key naive pluripotency-promoting transcripts results in transcript destabilization (By similarity. M6A regulates spermatogonial differentiation and meiosis and is essential for male fertility and spermatogenesis (By similarity. M6A also regulates cortical neurogenesis: m6A methylation of transcripts related to transcription factors, neural stem cells, the cell cycle and neuronal differentiation during brain development promotes their destabilization and decay, promoting differentiation of radial glial cells (By similarity.,METTL14,hMETTL14,Cell Biology & Developmental Biology,METTL14

Molecular Weight: 52 kDa

Gene ID: 57721

UniProt: Q9HCE5