TCR gamma/delta antibody

Catalog No. ABIN611633

Product Details anti-TCR gamma/delta Antibody

Target: TCR gamma/delta (T-Cell Receptor gamma/delta (TCR gamma/delta))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This TCR gamma/delta antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: The mouse monoclonal antibody V65 recognizes an extracellular epitope of TCR gamma/delta, the subtype of T cell receptor expressed mainly in epithelial tissues and at the sites of infection.

Cross-Reactivity (Details): Rat

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: TCR alpha/beta-negative CD3-positive rat T cell hybridoma III.89.1.4 line

Clone: V65

Isotype: IgG1 kappa

Application Details

Application Notes: Flow cytometry: Recommended dilution: 1 μg/mL.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-TCR gamma/delta antibody (ABIN611633)

De Bernardis, Lucciarini, Boccanera, Amantini, Arancia, Morrone, Mosca, Cassone, Santoni: "Phenotypic and functional characterization of vaginal dendritic cells in a rat model of Candida albicans vaginitis." in: Infection and immunity, Vol. 74, Issue 7, pp. 4282-94, (2006) (PubMed).

van den Brandt, Kwon, Hünig, McPherson, Reichardt: "Sustained pre-TCR expression in Notch1IC-transgenic rats impairs T cell maturation and selection." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 174, Issue 12, pp. 7845-52, (2005) (PubMed).

Moghaddami, Cleland, Mayrhofer: "MHC II+ CD45+ cells from synovium-rich tissues of normal rats: phenotype, comparison with macrophage and dendritic cell lineages and differentiation into mature dendritic cells in vitro." in: International immunology, Vol. 17, Issue 8, pp. 1103-15, (2005) (PubMed).

Xystrakis, Dejean, Bernard, Druet, Liblau, Gonzalez-Dunia, Saoudi: "Identification of a novel natural regulatory CD8 T-cell subset and analysis of its mechanism of regulation." in: Blood, Vol. 104, Issue 10, pp. 3294-301, (2004) (PubMed).

Henriksson, Helgeland, Midtvedt, Stierna, Brandtzaeg: "Immune response to Mycoplasma pulmonis in nasal mucosa is modulated by the normal microbiota." in: American journal of respiratory cell and molecular biology, Vol. 31, Issue 6, pp. 657-62, (2004) (PubMed).

Hosea, Rector, Taylor: "Zinc-deficient rats have fewer recent thymic emigrant (CD90+) T lymphocytes in spleen and blood." in: The Journal of nutrition, Vol. 133, Issue 12, pp. 4239-42, (2003) (PubMed).

Giezeman-Smits, Jonges, Chambers, Brisette-Storkus, Van Vlierberghe, Van Eendenburg, Eggermont, Fleuren, Kuppen: "Novel monoclonal antibodies against membrane structures that are preferentially expressed on IL-2-activated rat NK cells." in: Journal of leukocyte biology, Vol. 63, Issue 2, pp. 209-15, (1998) (PubMed).

Target Details for TCR gamma/delta

Target: TCR gamma/delta (T-Cell Receptor gamma/delta (TCR gamma/delta))

Alternative Name: TCR gamma/delta (TCR gamma/delta Products)

Background: The antigen-specific T cell receptor (TCR) is composed of either alpha and beta subunit, or gamma and delta subunit. Majority of T cells present in the blood, lymph and secondary lymphoid organs express TCR alpha/beta heterodimers, whereas the T cells expressing TCR gamma/delta heterodimers are localized mainly in epithelial tissues and at the sites of infection. The subunits of TCR heterodimers are covalently bonded and in the endoplasmic reticulum they associate with CD3 subunits to form functional TCR-CD3 complex. Lack of expression of any of the chains is sufficient to stop cell surface expression.,TCRG/D