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CD28 antibody

CD28 Reactivity: Human FACS, ELISA, Func Host: Mouse Monoclonal ANC28-1-5D10 unconjugated F(ab')2 fragment

Catalog No. ABIN610016

Datasheet as PDF

Target See all CD28 Antibodies

CD28

Fragment

F(ab')2 fragment
Reactivity
166

113

48

37

23

16

12

12

7

7

6

5

1

1

1

1
Human
Host
132

70

28

16

12

3

2
Mouse
Compatible Secondaries
Clonality
192

65

3
Monoclonal
Conjugate
94

33

30

20

14

5

4

4

4

4

3

3

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1
This CD28 antibody is un-conjugated
Application
207

90

54

47

38

35

28

26

21

17

13

7

6

6

4

4

4

2

2

2

2

1

1

1

1

1

1
Flow Cytometry (FACS), ELISA, Functional Studies (Func)

Specificity

CD28 expressed on T cells and also on BHK cells transfected with human CD28. This antibody has been characterized as a superagonist.

Purification

Gel filtration

Immunogen

Recombinant Human CD28 and HPB-MLT human tumor cells.

Type of Immunogen: Recombinant protein

Clone

ANC28-1-5D10

Isotype

IgG1 kappa

Top Product

Discover our top product CD28 Primary Antibody
anti-CD28 (CD28) (AA 1-120) antibody
CD28 Reactivity: Human, Mouse, Rat FACS, WB, ELISA, IHC (fro), IF (p), IHC (p), IF (cc) Host: Rabbit Polyclonal unconjugated

anti-CD28 (CD28) antibody
CD28 Reactivity: Human, Non-Human Primate FACS, WB, IP, IHC (fro), ICC Host: Mouse Monoclonal CD28-2 unconjugated

anti-CD28 (CD28) antibody (FITC)
CD28 Reactivity: Human, Non-Human Primate FACS Host: Mouse Monoclonal CD28-2 FITC

anti-CD28 (CD28) antibody
CD28 Reactivity: Human, Non-Human Primate FACS, WB, IP, Func, IHC (fro), ICC Host: Mouse Monoclonal CD28-2 unconjugated

anti-CD28 (CD28) (AA 19-152) antibody
CD28 Reactivity: Human FACS, ELISA Host: Rabbit Monoclonal DM61 unconjugated Recombinant Antibody

anti-CD28 (CD28) antibody
CD28 Reactivity: Rat FACS, IP Host: Mouse Monoclonal JJ319 unconjugated

anti-CD28 (CD28) antibody (PE)
CD28 Reactivity: Rat FACS Host: Mouse Monoclonal JJ319 PE

anti-CD28 (CD28) (C-Term) antibody
CD28 Reactivity: Human, Mouse, Rat WB, IHC Host: Rabbit Polyclonal unconjugated

anti-CD28 (CD28) antibody (PerCP)
CD28 Reactivity: Human, Non-Human Primate FACS Host: Mouse Monoclonal CD28-2 PerCP

anti-CD28 (CD28) antibody (APC)
CD28 Reactivity: Human, Non-Human Primate FACS Host: Mouse Monoclonal CD28-2 APC

Application Notes

Approved: ELISA, Flo, Func

Usage: Function: ANC28.1 stimulates expression of IL-2 from CD28+ cells. Divalent ANC28.1 can induce Antigen Independent T cell proliferation. In combination with anti-CD81 mAb, it can prime naive T cells to acquire effector function independent of TCR ligation. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.

Not recommended for: WB

Comment

Target Species of Antibody: Human

Restrictions

For Research Use only
Format

Liquid

Concentration

Lot specific

Buffer

50 mM sodium phosphate,  pH 7.5, 100 mM potassium chloride, 150 mM sodium chloride

Handling Advice

Do not freeze. Open under aseptic conditions.

Storage

4 °C

Storage Comment

Store at 4°C. Do not freeze. Open under aseptic conditions.
Target

CD28

Alternative Name

CD28 (CD28 Products)

Synonyms

Tp44 antibody, CD28RNA antibody, cd28 antibody, CD28 molecule antibody, CD28 antigen antibody, Cd28 molecule antibody, CD28 molecule L homeolog antibody, CD28 antibody, Cd28 antibody, cd28.L antibody

Background

Name/Gene ID: CD28

Synonyms: CD28, CD28 antigen, CD28 antigen (Tp44), Tp44, CD28 Molecule

Gene ID

940

UniProt

P10747

Pathways

TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response