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CD11b antibody (AA 500-600) (Biotin)

ITGAM Reactivity: Human, Mouse IHC, WB, IHC (p) Host: Rabbit Polyclonal Biotin

Catalog No. ABIN608931

Datasheet as PDF

Target See all CD11b (ITGAM) Antibodies

CD11b (ITGAM) (Integrin alpha M (ITGAM))
Binding Specificity
16

14

10

7

6

6

5

4

4

3

3

3

3

3

3

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 500-600
Reactivity
260

163

64

34

20

19

17

14

11

10

7

4

4

2

2

1
Human, Mouse
Host
142

106

104

2

1

1
Rabbit
Compatible Secondaries
Clonality
258

95

1
Polyclonal
Conjugate
120

39

29

27

21

10

7

7

5

5

5

5

4

4

4

3

3

3

3

3

3

3

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1
This CD11b antibody is conjugated to Biotin
Application
267

83

79

78

59

46

37

22

21

19

14

14

7

6

5

5

4

3

2

2

2

1

1

1
Immunohistochemistry (IHC), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity

Recognizes CD11b/c. Using macrophage lysates, a doublet is seen ~95 and 103kD. A weaker doublet is also seen ~130 and 160kD. Species cross-reactivity: human and mouse. Cellular Localization: monocytes and granulocytes.

Purification

Immunoaffinity purified

Immunogen

A synthetic peptide made to an internal region (aa500-600) of the mouse CD11 (b/c) protein.

Type of Immunogen: Synthetic peptide

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ITGAM Reactivity: Human IHC, WB, IF Host: Rabbit Polyclonal unconjugated

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ITGAM Reactivity: Human, Mouse, Rat FACS, WB, IHC (fro), ELISA, IHC (p), IF (cc), IF (p) Host: Rabbit Polyclonal unconjugated

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ITGAM Reactivity: Rat IHC, WB, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-Integrin alpha M (ITGAM) antibody (FITC)
ITGAM Reactivity: Human FACS Host: Mouse Monoclonal MEM-174 FITC

anti-Integrin alpha M (ITGAM) antibody (Biotin)
ITGAM Reactivity: Human FACS, IP Host: Mouse Monoclonal MEM-174 Biotin

anti-Integrin alpha M (ITGAM) antibody (PE)
ITGAM Reactivity: Human FACS Host: Mouse Monoclonal MEM-174 PE

anti-Integrin alpha M (ITGAM) (AA 623-728) antibody
ITGAM Reactivity: Human ELISA, ICC Host: Mouse Monoclonal 3A10H5 unconjugated

anti-Integrin alpha M (ITGAM) (AA 941-1074) antibody
ITGAM Reactivity: Human IHC, WB, StM Host: Mouse Monoclonal ITGAM-3340 unconjugated

Application Notes

Approved: IHC, IHC-P (5 - 10 μg/mL), WB (2 μg/mL)

Usage: Suitable for use in Western Blot, Immunohistochemistry. Western Blot: 2 μg/mL. Immunohistochemistry (regular/paraffin): 5-10 μg/mL. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment

Target Species of Antibody: Mouse

Restrictions

For Research Use only
Format

Liquid

Concentration

Lot specific

Buffer

Tris-glycine, 150 mM sodium chloride, 0.05 % sodium azide. Labeled, Biotin

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice

Avoid repeated freezing and thawing

Storage

4 °C,-20 °C

Storage Comment

May be stored at 4°C for short-term only. For long-term storage and to avoid freeze-thaw cycles, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 1 year at -20°C.
Target

CD11b (ITGAM) (Integrin alpha M (ITGAM))

Alternative Name

CD11b + C (ITGAM Products)

Background

This antibody detects: CD11b + c

Pathways

Apoptosis, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation