Recognizes a small epitope, termed Pk, present on the P/V proteins of the paramyxovirus, SV5. Has been used to detect recombinant proteins, some of which include transmembrane and secreted proteins, which have been tagged with this epitope. Usually, a 14 amino acid tag has been added to the recombinant proteins, although a smaller epitope of 9 amino acids (that as a peptide inhibit the binding of the monoclonal antibody to its native protein) has also been successfully used. The 14 amino acid epitope is, gly lys pro ile pro asn pro leu leu gly leu asp ser thr. (The 9 amino acid epitope is underlined.
Usage: Western Blot:1:1000-1:5000. ELISA. Immunohistochemistry (frozen). The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Restrictions
For Research Use only
Format
Liquid
Concentration
Lot specific
Buffer
IgG conjugated to Biotin. Supplied as a liquid, 0.1 % sodium azide
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Aliquot to Avoid repeated freezing and thawing.
Storage
4 °C,-20 °C
Storage Comment
May be stored at 4°C for short-term only. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Aliquots are stable for at least 1 year.