Nuclei and Chromosomes, Histone Specific antibody

Catalog No. ABIN600034

Product Details

Target: Nuclei and Chromosomes, Histone Specific

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: Histone specific, reacting preferentially with histone H1, a nuclear protein necessary for the condensation of nucleosome chains into higher order structures. This antibody stains nuclei of all human cell types and also stains chromosomes diffusely in metaphase cells. Intense diffuse staining is obtained in interphase cells. Species cross-reactivity: rat.

Purification: Ammonium sulfate precipitation

Immunogen: Native.

Type of Immunogen: Native protein

Isotype: IgG2a

Application Details

Application Notes: Approved: ELISA, IF (1:20 - 1:30), IHC, IHC-Fr, IHC-P, WB (1:20 - 1:30)

Usage: Suitable for use in ELISA, Immunofluorescence, Immunohistochemistry (frozen and paraffin), and Western Blot. Immunofluorescence: 1:20-1:30.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Aliquot to Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: May be stored at 4°C for short-term only. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Aliquots are stable for at least 1 year.

Target Details

Target: Nuclei and Chromosomes, Histone Specific